Imagine you are working in a lab that is developing a novelty rose that smells like a stinkhorn mushroom – called the “Grose.”   The gene that produces the odorous protein has been isolated from stinkhorn and recombinant DNA containing this gene, a promoter, and a gene that confers resistance to the antibiotic ampicillin has already been produced. After transforming your rose cells, describe the next 2 steps to verify that you have successfully produced this genius gag gift.

Q: You are studying a human gene, and try to express the protein in E. coli bacterial cells. To do…

A: Cloning is the process of creating a genetically identical copy of a biological entity. Cloning can…

Q: How does one performs a Southern blot and how one performs a western blot? Then, consider what a…

A: The transfer of biomolecules like protein, DNA, or RNA from gel to the carrier blotting membranes is…

Q: After much work the researcher maps the mutation in the mut1 plant to a locus on chromosome 2.  The…

A: CoB1 gene is a component of the ubiquinol- cytochrome c reductase complex that is part of the…

Q: Explain what epigenetic theory is.

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Q: The diagram shown represents the coding strand of the myosin gene. Mutations in myosin can lead to…

A: mRNA  The short chain of RNA molecule that is made by the process of transcription. And translated…

Q: reproductive cloning

A: Apart from the natural reproduction of various organisms in our ecosystem, the advent of technology…

Q: Mutation in the operator that reduces the affinity of the operator for the repressor protein…

A: Lac operon is an inducible operon. When lactose is present in medium, then lac operon starts.

Q: this is an example of biotechnology: In order to increase the yield of oil from canola, research…

A: Biotechnology is the branch of science that deals with the production of various products by using…

Q: Regulatory regions for genes can also be cloned using molecular-genetic techniques, allowing…

A: Yes, Regulatory regions of genes can be cloned using molecular genetic techniques for studying gene…

Q: The introduction of genes into plants is a common practice that has generated not only a host of…

A: No, the tumor-inducing genes are removed from the plasmid, eliminating the threat of tumor…

Q: The statement below may be true or false. If it is true then type "true" in the box below. If it is…

A: DNA that is silenced can not bound by transcrpition fators

Q: During experimental RNAi, how does the researcher affect expression of a target gene? Group of…

A: Introduction: In the process of RNA interference(RNAi) double-stranded RNA molecule suppresses the…

Q: You are an expert in DNA-repair mechanisms. You receive a sample of a human cell line derived from…

A: In normal conditions, the cells are capable of fixing the damages caused to DNA before problems…

Q: magine you are working in a lab that is developing a novelty rose that smells like a stinkhorn…

A: Introduction Recombinant DNA technology is advanced technology to produce hybrids plants and animals…

Q: You are interested in finding out in which organ GTF2H5 is highly expressed experimentally. Describe…

A: Recombinant DNA also written as rDNA is formed by joining or combining the DNA from different…

Q: You run a Western blot to detect Ras in sampe A and sample B. You find that sample B gives a darker…

A: First of all, Western Blot is a technique which is used to detect the expression of protein from…

Q: Igf-2 is maternally imprinted gene in mammals and in Martians. A Martian couple, B@sb&t (female –…

A: The transcription or expression of a gene is where the information in a gene is used to make a…

Q: Four terms and statements related to gene regulatlon Match the correct term to each of the four…

A: According to the question, four terms and statements are related to gene regulation in bacteria are…

Q: Ye are helping clinicians to diagnose a patient with a rare liver condition. After some preliminary…

A: PHGDH is an enzyme that stands for Phosphoglycerate Dehydrogenase. This enzyme plays an important…

Q: How will this modified cro protein interact with the three OR sites, and how would cro expression be…

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Q: According to the scenario shown, how many segments of DNA (one, two, or three) are removed during…

A: Genetics is the branch of biology which deals with genes, heredity, and genome in the organism.…

Q: How would you use a ChIP (chromatin immunoprecipitation) to show that when RNA pol II is bound at…

A: Chromatin immunoprecipitation is a technique used for the investigation of DNA (deoxyribonucleic…

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A: The molecular cloning is a technique of production of transgenic organisms. Here one gene is…

Q: I am a Physician Scientist and have a patient that presents with colorectal cancer. I decide to…

A: Given the scenario you've described, where a novel SNP leads to a premature stop codon in a gene…

Q: From gene expression studies on cancer, you discovered a hypothetical gene called ABCG2 that is…

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Q: A researcher studying epigenetic changes induced by exercise had participants pedal a stationary…

A: Introduction DNA is the molecular structure that is composed of a pair of polynucleotide chains and…

Q: After decades of work, Dr. Ricky M. isolated a small amount of attractase—an enzyme that produces a…

A: A gene is the essential physical and functional unit of heredity. Genes are comprised of DNA. A…

Q: Itransferases and analyze the cells after they at do you expect to find in the promoter of gene X?…

A: Hematopoietic stem cells (HSCs) resides within the bone marrow, which serves as niche for the…

Q: protein Menchie is conserved from cats to humans. The region upstream of the menchie gene has three…

A: This experiment is all about the gene expression process. Gene expression is a process in which gene…

Q: In Figure 13-5, two different methods are illustrated forvisualizing gene expression in developing…

A: Gene expression is the very important phenomenon which is almost occurs in every living organisms.…

Q: By whole-exome sequencing, you have identified an early termination mutation in KLHL4 in a human…

A: "Hi, since you have posted more than one question, we will be answering the first two for you.…

Q: Woolly mammoths have been extinct for about 4,000 years, but we often find their well-preserved…

A: Somatic cell nuclear transfer (SCNT) is a technique in which the viable embryo is developed from egg…

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A: Drosophila Cryptochrome -- Drosophila Cryptochrome is a blue - light sensitive protein which…

Q: Cap, EA1, and Sap are all genes and are also proteins. For each gene, what gene product is encoded…

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Q: Dolly is the first mammal to have been successfully cloned from an adult cell Which of the following…

A: A cell is the basic unit of life.  All living creatures are made up of cells, ranging from…

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Q: Your identify a number of mutants and start to characterize the problems in their mismatch repair…

A: Hi, Thanks For Your Question.  Answer : The Answer Is Given Below In Table Form For Better…

Q: Several DNA coding for different proteins, CRISPR, or siRNAs against different genes were expressed…

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Q: You conduct an experiment to study the expression of the S protein through the tissues of your…

A: The experiment is conducted on the Arabidopsis thaliana which is a common model plant used in…

Q: Discuss the steps you would proceed after selecting your E. coli strain to prepare competent cells…

A: c) We can select the E. coli DH5α cells to prepare competent cells for cloning. The steps for the…

Q: Your friend sends you two cancerous cell lines to examine and detérmihe posi mutations. The results…

A: Mutations are alterations in the DNA sequence of an organism. Small changes, such as adding or…

Q: How does one perform a Southern blot and how does one performs a western blot. What can a Southern…

A: Southern blotting is the procedure that requires the transfer of separated fragments of DNA (DNA) to…

Q: Part 2. Which lane or lanes on the gel could be the result of Mutation B? lane 1 lane 2 lane 3 lane…

A: Myosin, a motor protein primarily involved in muscle contraction, undergoes alterations in its…

Q: You were sequencing a region of 200 kb in Effrenium voratum (a member of the family Symbiodiniaceae)…

A: Insulators are the boundary elements that block the activating impact of distal enhancers.…