ou are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm^2 and when confluent contains 2 x 10^7 cells. Question: You seed a culture at 8 am on Monday with 5 x 10^5 cells. Assume all survive, a generation time of 18 hours and all cells are actively dividing. When would you expect them to be confluent? A) 7:24 AM on Saturday B) 8 pm on Tuesday C) 8 am on Tuesday D) 9 pm on Thursday E) 12:48 pm on Wednesday F) They won't grow that long

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm^2 and when confluent contains 2 x 10^7 cells.

Question: You seed a culture at 8 am on Monday with 5 x 10^5 cells. Assume all survive, a generation time of 18 hours and all cells are actively dividing. When would you expect them to be confluent?

A) 7:24 AM on Saturday

B) 8 pm on Tuesday

C) 8 am on Tuesday

D) 9 pm on Thursday

E) 12:48 pm on Wednesday

F) They won't grow that long

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