You need a buff er at pH 7.5 for use in purifying a protein at 4°C. You have chosen Tris, pK 8.08, ΔH° = 50 kJ · mol−1. You carefully make up 0.01 M Tris buffer, pH 7.5 at 25°C, and store it in the cold to equilibrate it to the temperature of the purifi cation. When you measure the pH of the temperature-equilibrated buff er it has increased to 8.1. What is the explanation for this increase? How can you avoid this problem?

Q: Protein concentration standards 0.6 0.5 0.4 0.3 0.2 0.1 0.5 1 1.5 2.5 Albumin concentration (mg/mL)…

A: Proteins are essential macromolecules/polymers that are composed of peptide bound amino acids. They…

Q: If I want to make a 100mL buffer that has 2% SDS, 100mM tris pH 8, 10mM EDTA, and proteinase K…

A: Introduction: Buffer is defined as a solution that resists change in pH on the addition of acid or…

Q: (MB = methylene blue) What is the concentration of the sample of the substance with the reporter…

A: Here the absorbance of the methylene blue sample is given to be: 0.40 at 595 nm. According to the…

Q: if you had a protein sample solution of unknown concentration which gave an absorbance of 0.992…

A: Answer:- The standard curve gives the absorption value of aliquots having the substance of known…

Q: The order is for Moxam 5 gm IM every 8 hours.  On hand is 10 gram of Moxam with the following…

A: Medication order is the terms used to define the directions for a drug needed to be given to the…

Q: Pretend the results below were obtained for water from your well. The 10 mL tubes are Full Strength,…

A: Most Probable Number or MPN method is a way to estimate the number of microorganisms in the water…

Q: You are running a size exclusion column to purify your 25 kDa protein from a lysate mixture. The…

A: Size exclusion column chromatography: As a function of their respective sizes, molecules are…

Q: What rate in mL/hr of Isosource VHN would provide 1.6 g protein/kg/day if run for 24 hours for a…

A: Protein is building blocker food item for the body. It is used for the muscle formation, tendons,…

Q: The Thermal conductivity of rock salt measured at a temperature of 500 degrees C what value would be…

A: thermal conductivity at 500°C would be about 0.2 W/m°C less than the thermal conductivity at 400°C…

Q: The pH of an aqueous solution containing fermentation nutrients with low buffering capacity should…

A: Fermentation is a critical process in biotechnology and the food industry that involves microbes…

Q: Given 10 gram of vitamin C (E=0.18), we can make ml of isotonic Vitamin C solution.

A: Isotonic solution has the same water concentration and solute concentration as in the cytoplasm of…

Q: Compute for the lactic acid content of these samples given the values below. Show your solution…

A: The amount of substance present in the mixture of substances can be defined in terms of…

Q: You obtained the following raw data when setting up a Biuret standard curve:   BSA (mg/ml)…

A: Biuret is a compound that formed when urea is warmed, which makes two urea particles to join. Urea…

Q: The aliquot method was used to obtain 8 mg of a drug with a pr

A: Introduction:- the amount of the drug that is weighed that is within the degree of accuracy that…

Q: can you show how to calculate the amount of two components for the required volume of a buffer with…

A: Given Values: Concentration of Tris = 0.25 M Concentration of Glycine = 1.92 M Concentration of SDS…

Q: It is recommended that children should have fluoride supple- ments for their teeth if the fluoride…

A: To convert parts per million (ppm) to micrograms per liter (µg/L), we need to know the molar mass of…

Q: You have a 100X stock of a buffer. You need 100 ml of 1X buffer.  How much buffer solution do you…

A: Stock solutions of the standard concentration are present in the laboratories. Using these stock…

Q: if you have Quinoa Serving size -170 grams Carbohydrates – 109 grams calculate the DV % for these…

A: DV% is how much of a nutrient is present in a single serving of a packaged food or a dietary…

Q: Figure 3: ● • . ● KDa ● 97.4 66.2 45.0 ● 31.0 21.5 14.4 S-1 p-1 2.8 2-d S.3 p-3 This figure was…

A: The study of protein behavior under different conditions is a fascinating aspect of molecular…

Q: To prepare 500 mL of a 1X TAE buffer solution, we need to mix solution (so 25 times more…

A: TAE is a standard buffer for the preparation and operation of DNA agarose gels. TBE provides…

Q: How would you make wash buffer with 0.05M Tris containing 0.05M NaCl in 200ml at pH of 8.0

A: A solution is a mixture of one or more than one solute mixed in a solvent. The molarity of the…

Q: [2] Calculate the pH of the arginine solution after the addition of Oml, 5ml of 0.1M NaOH and…

A: A method employed in laboratories for calculating an amino acid's pKa values and isoelectric point…

Q: You have a stock solution of protein that you would like to dilute. How much stock protein do you…

A: Dilution is a process to lower the concentration the concentration of a solute by the addition of…

Q: We usually say that a perfect buffer has its pH equal to its pKa. Give an example of a situation in…

A: A buffer is a solution that, when adding an acid or basic components, may resist pH changes. Limited…

Q: Calculate the appropriate volume (in µL) of 3X loading buffer that should be added to 24.0 µL of a…

A: Dilution factor (DF) can be expressed both as the ratio of concentrations and as ratio of volumes.…

Q: Total Protein Determination Spectroscopy Values CREATE CALIBRATION CURVE? DRAWN CONCENTRATION AND…

A: The protein estimation in a sample can be done by analyzing and measuring the absorbance of the…

Q: In solubility test, why do we need to put the lipid samples in a direct heat after adding ethanol?

A: In solubility test, why do we need to put the lipid samples in a direct heat after adding ethanol?…

Q: calculate the [PNP] in μM for each of these samples.

A: Molarity is way of representing the concentration of a solution. Molarity is number of moles of…

Q: Match the common laboratory scenarios to the correct buffer required using the guide below.…

A: Buffer  It is defined as a solution that have the ability to resist change in pH upon the addition…

Q: You have a sample at 50 ng/ul and you would like to load 400ng of this sample on a lane of an…

A: Agarose gel electrophoresis is a technique to study the features of genetic material and proteins.…

Q: 67. Purified water contains not more than 10 ppm of total solids. Express this concen- tration as a…

A: PPM is an abbreviation for "parts per million" and it also can be expressed as milligrams per liter…

Q: separating gel. The separating gel is 12% acrylamide, 0.1% SDS, 0.375 M Tris-Cl, pH

A: Introduction SDS ( sodium dodcyl sulfate) is an anionic detergent which is negatively charged. And…

Q: The authors of this paper also tested another minimal media (Media C) which is exactly the same as…

A: Media C has a pH of 7.4 and media B has a pH of 7.1 qhich means that C is more basic than B and thus…

Q: Calculate the appropriate volume (in µL) of 9X loading buffer that should be added to 46.0 µL of a…

A: Agarose gel electrophoresis is a laboratory technique that is used for the separation of nucleic…

Q: You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis.…

A: TAE buffer used in agarose gel electrophoresis for the seperation of DNA and RNA.

Q: How many mLs should be removed from your dextrose 70% bag?

A: Answer: The mass of dextrose present in 400 mL of solution is 280 g.

Q: Why is the pH of a 0.1 M solution of acetic acid in water higher than that of a 0.1 M solution of…

A: pH pH is a scale used to measure the basicity or acidity of an aqueous solution. Those solutions…

Q: Lipids from an organic sample are extracted separately by column chromatography using the ff…

A: The objective of the question is to identify the possible lipids present in each eluate based on the…

Q: You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule,…

A: Standard calibration curves are plotted by taking concentration of the solution in x axis and…

Q: Laemmli buffer, also referred to as SDS-reducing buffer, is added to each of the samples. It is a…

A: Buffer is defined as any solution that resists small change in its pH. Laemmli buffer is formulated…