We usually say that a perfect buffer has its pH equal to its pKa. Give an example of a situation in which it would be advantageous to have a buffer with a pH 0.5 unit higher than its pKa.
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We usually say that a perfect buffer has its pH equal to its pKa. Give an example of a situation in which it would be advantageous to have a buffer with a pH 0.5 unit higher than its pKa.
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- Which of the following is TRUE if pH is higher than the pka of the buffer? O [weak acid] approximately 0 O [conjugate base] = [weak acid] [conjugate base] > [weak acid] O [conjugate base] < [weak acid]If 4 mL of 1 M NaOH is added to 100 mL buffer, would it still be a usable buffer according to the conventions? Explain why or why not.Define the following:- pH- Buffer- pKa
- Suppose you wanted to make a buffer of exactly pH 7.00 using KH2PO4 and Na2HPO4. If the final solution was 0.1 M in KH2PO4, what concentration of Na2HPO4 would you need?The glass electrode used in commercial pH meters gives an electrical response proportional to the concentration of hydrogen ion. To convert these responses to a pH reading, the electrode must be calibrated against standard solutions of known H+ concentration. Determine the weight in grams of sodium dihydrogen phosphate (NaH2PO4 · H2O; FW 138) and disodium hydrogen phosphate (Na2HPO4 ; FW 142) needed to prepare 1 L of a standard buffer at pH 7.00 with a total phosphate concentration of 0.100 M . See Problem 24 for the pKa values of phosphoric acid.Make a list of the five most critical qualities of an effective extraction buffer. Please provide a brief description of each.
- You need to prepare an acetate buffer of pH 5.43 from a 0.621 M acetic acid solution and a 2.95 M KOH solution. If you have 730 mL of the acetic acid solution, how many milliliters of the KOH solution do you need to add to make a buffer of pH 5.43? The pKa of acetic acid is 4.76. Be sure to use appropriate significant figures.I want to make a buffer solution containing 1 M glycene pH = 10 (Na+), 1 mM ZnCl2 , 1 mM MgCl2. I have the stock solutions 1 M glycene, 1 M MgCl2, 1 M ZnCl2, and 10 M NaOH. My final volume of buffer solution needs to be 250ml. how much (in ml) of each solution should i put into my buffer?It is possible to make a buffer that functions well near pH7 using citric acid, which contains only carboxylate groups. Explain.
- 3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mLAt what pH does a lysine solution exhibit the highest buffering capacity? (Lysine pKas: pk1 = 2.2, pK2 = 8.95, pK3 = 10.5) %3D O рH 12.1 О рH 9.72 O pH 5.67 pH 8.95Another characteristic of modern buffers such as HEPES is that their pH changes little with changes in temperature. Why is this desirable?