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Under the restrictive conditions a conditional mutation expresses its mutant phenotype and under the permissive conditions expresses the normal phenotype. First type of conditional mutation is a temperature-sensitive mutation, that expresses the mutant phenotype only at certain temperatures. Strains of E.coli have been isolated that contain temperature-sensitive mutations in genes encoding different components of the replication machinery. In the each of these strains, the protein produced by the mutated gene is nonfunctional under the restrictive conditions. You grow these strains under the permissive conditions and then abruptly switch them to the restrictive conditions. After one round of replication under the restrictive conditions, you isolate DNA from each strain and analyze it. What characteristics would you expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes each of the following proteins?

Under the restrictive conditions a conditional mutation expresses its mutant phenotype and under the permissive conditions expresses the normal phenotype. First type of conditional mutation is a temperature-sensitive mutation, that expresses the mutant phenotype only at certain temperatures. Strains of E.coli have been isolated that contain temperature-sensitive mutations in genes encoding different components of the replication machinery. In the each of these strains, the protein produced by the mutated gene is nonfunctional under the restrictive conditions. You grow these strains under the permissive conditions and then abruptly switch them to the restrictive conditions. After one round of replication under the restrictive conditions, you isolate DNA from each strain and analyze it. What characteristics would you expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes each of the following proteins?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Under the restrictive conditions a conditional mutation expresses its mutant phenotype and under the permissive conditions expresses the normal phenotype. First type of conditional mutation is a temperature-sensitive mutation, that expresses the mutant phenotype only at certain temperatures. Strains of E.coli have been isolated that contain temperature-sensitive mutations in genes encoding different components of the replication machinery. In the each of these strains, the protein produced by the mutated gene is nonfunctional under the restrictive conditions. You grow these strains under the permissive conditions and then abruptly switch them to the restrictive conditions. After one round of replication under the restrictive conditions, you isolate DNA from each strain and analyze it. What characteristics would you expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes each of the following proteins? 1. DNA ligase 2. DNA polymerase I 3. DNA polymerase III 4. Primase 5. Initiator protein
Transcribed Image Text:Under the restrictive conditions a conditional mutation expresses its mutant phenotype and under the permissive conditions expresses the normal phenotype. First type of conditional mutation is a temperature-sensitive mutation, that expresses the mutant phenotype only at certain temperatures. Strains of E.coli have been isolated that contain temperature-sensitive mutations in genes encoding different components of the replication machinery. In the each of these strains, the protein produced by the mutated gene is nonfunctional under the restrictive conditions. You grow these strains under the permissive conditions and then abruptly switch them to the restrictive conditions. After one round of replication under the restrictive conditions, you isolate DNA from each strain and analyze it. What characteristics would you expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes each of the following proteins? 1. DNA ligase 2. DNA polymerase I 3. DNA polymerase III 4. Primase 5. Initiator protein
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