Homework Help is Here – Start Your Trial Now!
Science
Biology
Under the restrictive conditions a conditional mutation expresses its mutant phenotype and under the permissive conditions expresses the normal phenotype. First type of conditional mutation is a temperature-sensitive mutation, that expresses the mutant phenotype only at certain temperatures. Strains of E.coli have been isolated that contain temperature-sensitive mutations in genes encoding different components of the replication machinery. In the each of these strains, the protein produced by the mutated gene is nonfunctional under the restrictive conditions. You grow these strains under the permissive conditions and then abruptly switch them to the restrictive conditions. After one round of replication under the restrictive conditions, you isolate DNA from each strain and analyze it. What characteristics would you expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes each of the following proteins? 1. DNA ligase 2. DNA polymerase I 3. DNA polymerase III 4. Primase 5. Initiator protein

Under the restrictive conditions a conditional mutation expresses its mutant phenotype and under the permissive conditions expresses the normal phenotype. First type of conditional mutation is a temperature-sensitive mutation, that expresses the mutant phenotype only at certain temperatures. Strains of E.coli have been isolated that contain temperature-sensitive mutations in genes encoding different components of the replication machinery. In the each of these strains, the protein produced by the mutated gene is nonfunctional under the restrictive conditions. You grow these strains under the permissive conditions and then abruptly switch them to the restrictive conditions. After one round of replication under the restrictive conditions, you isolate DNA from each strain and analyze it. What characteristics would you expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes each of the following proteins? 1. DNA ligase 2. DNA polymerase I 3. DNA polymerase III 4. Primase 5. Initiator protein

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Topic Video
Question
Under the restrictive conditions a conditional mutation expresses its mutant phenotype and under the permissive conditions expresses the normal phenotype. First type of conditional mutation is a temperature-sensitive mutation, that expresses the mutant phenotype only at certain temperatures. Strains of E.coli have been isolated that contain temperature-sensitive mutations in genes encoding different components of the replication machinery. In the each of these strains, the protein produced by the mutated gene is nonfunctional under the restrictive conditions. You grow these strains under the permissive conditions and then abruptly switch them to the restrictive conditions. After one round of replication under the restrictive conditions, you isolate DNA from each strain and analyze it. What characteristics would you expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes each of the following proteins? 1. DNA ligase 2. DNA polymerase I 3. DNA polymerase III 4. Primase 5. Initiator protein
Transcribed Image Text:Under the restrictive conditions a conditional mutation expresses its mutant phenotype and under the permissive conditions expresses the normal phenotype. First type of conditional mutation is a temperature-sensitive mutation, that expresses the mutant phenotype only at certain temperatures. Strains of E.coli have been isolated that contain temperature-sensitive mutations in genes encoding different components of the replication machinery. In the each of these strains, the protein produced by the mutated gene is nonfunctional under the restrictive conditions. You grow these strains under the permissive conditions and then abruptly switch them to the restrictive conditions. After one round of replication under the restrictive conditions, you isolate DNA from each strain and analyze it. What characteristics would you expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes each of the following proteins? 1. DNA ligase 2. DNA polymerase I 3. DNA polymerase III 4. Primase 5. Initiator protein
Expert Solution
steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Mitochondrial mutations
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS