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Transport kinetics through protein transporters can be described using the language of Michaelis-Menten kinetics. The transported substance binding to its protein transporter is analogous to the substrate binding to its enzyme. Thus, KM and vmax values can be determined for protein transporters.   a. Use the line graph to estimate KM and vmax values for glucose uptake max by pericytes in the presence and absence of sodium ions. Show your work!   b. What do these values tell you about the binding of glucose in pericytes? Specifically, have the investigators demonstrated convincingly that an SGLT exists in pericytes? In endothelial cells? Use evidence from any of the preceding questions to support your answers.

Transport kinetics through protein transporters can be described using the language of Michaelis-Menten kinetics. The transported substance binding to its protein transporter is analogous to the substrate binding to its enzyme. Thus, KM and vmax values can be determined for protein transporters.   a. Use the line graph to estimate KM and vmax values for glucose uptake max by pericytes in the presence and absence of sodium ions. Show your work!   b. What do these values tell you about the binding of glucose in pericytes? Specifically, have the investigators demonstrated convincingly that an SGLT exists in pericytes? In endothelial cells? Use evidence from any of the preceding questions to support your answers.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transport kinetics through protein transporters can be described using the language of
Michaelis-Menten kinetics. The transported substance binding to its protein transporter is analogous to the substrate binding to its enzyme. Thus, KM and vmax values can be determined for protein transporters.
 
a. Use the line graph to estimate KM and vmax values for glucose uptake max by pericytes in the presence and absence of sodium ions. Show your work!
 
b. What do these values tell you about the binding of glucose in pericytes?
Specifically, have the investigators demonstrated convincingly that an
SGLT exists in pericytes? In endothelial cells? Use evidence from any of the preceding questions to
support your answers.
 
 
 
1/v, mg.hr/nmol -0.5 0.5 0.4 0.3 0.2 0.1 0 ☐ with Na+ ions 0.5 1 [Glucose]¹, mM 1.5 ■ without Na+ ions 2
Transcribed Image Text:1/v, mg.hr/nmol -0.5 0.5 0.4 0.3 0.2 0.1 0 ☐ with Na+ ions 0.5 1 [Glucose]¹, mM 1.5 ■ without Na+ ions 2
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