Hypothetical scenerio: When the membrane potential is 80mV across the digestive vacuoles (DV), inside positive, the Vmax for VF-6-2 efflux is 15pmol/h. However, when the membrane potential is 80mV inside negative, the Vmax of VF-6-2 efflux drops to near zero. Researchers suggest an H+ P-type ATPase is also in the membrane and is required for VF-6-2 efflux. Do these data support their suggestion? What type of transporter might PfCRT be? How can vanadate be used to potentially test their idea?
Hypothetical scenerio:
When the membrane potential is 80mV across the digestive vacuoles (DV), inside positive, the Vmax for VF-6-2 efflux is 15pmol/h. However, when the membrane potential is 80mV inside negative, the Vmax of VF-6-2 efflux drops to near zero. Researchers suggest an H+ P-type ATPase is also in the membrane and is required for VF-6-2 efflux.
Do these data support their suggestion? What type of transporter might PfCRT be? How can vanadate be used to potentially test their idea?
All cells have an electrical potential difference or membrane potential across their plasma membrane. Electric potential across the plasma membrane is a function of the ion concentration in the intracellular and extracellular space and the selective permeabilities of the ions. Ion concentration gradients across the plasma membrane and selective movements of ions along gradient creates a difference in electric potential or voltage across the plasma membrane. This is called membrane potential.
Step by step
Solved in 2 steps
