**UTSAse** is an enzyme that catalyzes reactions that produce bluish-orange saliva in roadrunners. UTSA scientists have discovered that dodecyl gallate inhibits this reaction. Using the Lineweaver-Burk plot data given, calculate the Vmax (no inhibitor y = 1.52x +1.51; in the presence of inhibitor y = 1.58x +4.27 where x = Optical Density-1 min-1) and Km (no inhibitor -1/Km = -1.0; presence of inhibitor -1/Km = -2.7) for UTSAse in the presence and absence of the inhibitor. Indicate which of the following statements are TRUE.A. In the absence of inhibitor, Km (mM) = 1.0. B. In the presence of inhibitor, Km (mM) = 0.37. C. In the absence of inhibitor, Vmax(OD min-1) = 1.51. D. In the presence of inhibitor, Vmax(OD min-1) = 4.27. E. Dodecyl gallate is a competitive inhibitor. F. Dodecyl gallate is a noncompetitive inhibitor. G. Dodecyl gallate is an uncompetitive inhibitor. H. Dodecyl gallate does not compete with the substrate for binding to the enzyme. I. Dodecyl gallate binds to free enzyme.

Lineweaver-Burk plot, also known as double-reciprocal plot, is the graph plotted for Lineweaver-Burk…

Answered: UTSASE is an enzyme that catalyzes…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Following is a Dixon plot for PNPP inhibition by inorganic phosphates. What answer choice shows the correct combination of the mode of inhibition and Ki of the EI complex? - Mixed inhibition, Ki= -21.2 uM - Mixed inhibition, Ki= 1/16.5 uM - Competitive inhibition, Ki= 16.5 uM - Competitive inhibition , Ki= -1/16.5 uM - None of the above
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