Run Number 1 1 2 2 3 3 4 4 5 5 6 6 Solute Na Ch Glucose Na Ch Glucose Na* Ch Glucose Na Ch Glucose Na Ch Glucose Na Ch Glucose Start Conc. L Start Conc. R (mm) (mm) 0.00 0.00 2.00 0.00 0.00 0.00 8.00 0.00 0.00 0.00 2.00 0.00 0.00 0.00 8.00 0.00 0.00 0.00 10.00 0.00 2.00 0.00 2.00 0.00 Carriers 500 500 500 500 700 700 700 700 100 100 700 700 Rate (mm/sec) 0.0000 0.0008 0.0000 0.0023 0.0000 0.0010 0.0000 0.0031 0.0000 0.0017 0.0042 0.0010 There was no Na Cl- added with 10mM of glucose; sodium is required for transport by the glucose carrier protein O There were fewer carrier proteins used with 10mM of glucose, so they reached saturation and transport slowed O The glucose carrier protein transports against a concentration gradient, so transport will be slower with a larger concentration gradient. There was not ATP added with 10mM of glucose; ATP is required for transport by the glucose carrier protein
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.


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