Test Results + or -? Points awarded CodeGlu – acidic end products yellow ______ _____+Glu – gas producedno ___-__ _____+Lys – lysine decarboxylase yellow ______ _____ = _____ Orn – ornithine decarboxylaseyellow ______ _____+H2S – iron sulfide producedno brown ______ _____+Ind – indole produced beige ______ _____ = _____ Adon – acidic end products red ______ _____+Lac – acidic end products red ______ _____+Arab – acidic end products yellow ______ _____ = _____ Sorb – acidic end productsyellow ______ _____+VP not neededDul – dulcitol fermented green ______ _____ = _____ PA – deamination forms precipitate no brown ______ _____+Urea – ammonia produced yellow ______ _____+Cit – citrate catabolized green ______ _____ = _____ The V-P test in chamber 9 is an optional test to be used in case two species had identical results. It is not included in determining the 5-digit code.`Final 5-digit code for the results: _____ _____ _____ _____ _____ 406 EXERCISE 51: RAPID IDENTIFICATION METHODSTABLE 51.2 EnteroPluri-Test Biochemical ReactionsIndicator ChangedTestCommentsFromToGLUAcid from glucoseRedYellowGas produced from fermentation of glucose trapped in this compartment, causingseparation of the waxGASLYSLysine decarboxylaseYellowPurpleORNOrnithine decarboxylaseYellowPurpleH2SFerrous ion reacts with sulfide ionsBlackINDKovacs reagent is added to the H2S/IND compartment to detect indoleBeigeRedADONAdonitol fermentationRedYellowLACLactose fermentationRedYellowARABArabinose fermentationRedYellowRedYellowSORBSorbitol fermentationVoges-Proskauer reagents detect acetoinBeigeRedV-PGreenYellowDULDulcitol fermentationPhenylpyruvic acid released from phenylalanine after its deamination combineswith iron salts to form a dark brown precipitatePABrownYellowPinkUREAAmmonia changes the pH of the mediumGreenBlueCITCitric acid used as a carbon sourceSource: Becton, Dickinson and Company, Sparks, MD 21152.Report. Add the circled numbers only within eachbracketed section, and enter this sum in the space5. Place the plastic lid on the tray and incubatethe strip for 24 hours at 35°C (FIGURE 51.4d).If the strip cannot be read after 24 hours, removethe strip from the incubator and refrigerate it.provided below the arrow. Read the five numbersin these spaces across as a five-digit number in theEnteroPluri-Test Codebook.*3. Dispose of the EnteroPluri-Test by placing it in theautoclave bag.PROCEDURE Third PeriodEnteroPluri-TestAPI 20E1. Interpret and record all reactions (TABLE 51.2) inthe Laboratory Report. Read all the other tests be-fore the indole and V-P tests, which follow.Indole test. Place the EnteroPluri-Test hori-zontally, with its flat surface pointing upward,and melt a small hole in the plastic film cov-ering the H,S/indole compartment by usinga warm inoculating loop. Add 3 to 4 drops ofKovacs reagent, and allow the reagent to con-tact the agar surface. A positive test is indi-cated by a red color within 10 seconds.b. V-P test. Add 3 drops of V-P reagent I and 2drops of V-P reagent II to the V-P compart-ment. A positive test is indicated by develop-ment of a red color within 20 minutes.1. Interpret and record all reactions (TABLE 51.3) inthe Laboratory Report. Read all the other tests be-fore the TDA, VP, and IND tests, which follow.a. TDA test. Add 1 drop of 10% ferric chloride.A positive test is brownish-red. Indole-positiveorganisms may produce an orange color; thisis a negative TDA reaction.b. V-P test. Add 1 drop of V-P reagent I and 1drop of V-P reagent II. A positive reaction pro-duces a red color (not pale pink) after 10 minutes.Indole test. Add 1 drop of Kovacs reagent. A redring after 2 minutes indicates a positive reaction.d. Nitrate eduction. Before adding reagents,look r ablies in the GLU tube. Bubbles in-a.с.dicae reracion of nitrate to N2. Add 2 drops2. Indicate each positive reaction by circling the num-ber appearing below the appropriate compartmentof the EnteroPluri-Test diagram in the Laboratoryof te regnt n and 2 drops of nitrate re-agen A positive eation (red) may take*BD Bioscience, www.bco.

Introduction: Microbial metabolic processes are complex but still, it permits the microbiologist to…

Answered: Test Results + or -? Points awarded…

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P