The ideal temperature for all enzymes is 98.6 degrees F False O True
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- Aerobic degradation of an organic compound by mixed cultureof organism in wastewater can be represented by following reaction. C3H6O3 + a O2 + b NH3 → c C5H7NO2 + d H2o + e CO2 A. Determine a, b, c, d and e, if YX/S = 0.4 d X/g S. B. Determine the yield coefficients YX/O2 and YX/NH3. C. Determine the degree of reductions for the substrate, bacteria and RQ for the organisms33333333333333333333333 Using the table below, differentiate the effect of two varying pH levels (as indicated by by the color) to the amylase enzyme. How does pH level affects the enzymatic reaction (enzyme-substrate complex)? Tube 1 Tube 2 Tube 3 Tube 4 Ingredients StarchAmylaseBuffer pH 7 StarchAmylaseBuffer pH 2 MaltoseWaterBuffer pH 7 StarchWaterBuffer pH 7 Color (1) (2) Orange BlueAn enzymatic reaction follows M-M kinetics with Vmax= 2.5 mol m-3s-1and Km = 5 mM.Calculate the time required for 50% conversion of the substrate in a batch reactor if theinitial substrate concentration is0.2 M.Show your calculation steps.
- -n f estion B I ut of question PF budy As you increase the amount of substrate in a reaction (while keeping the enzyme concentration the same): Select one: O A. The amount of products formed should decrease OB. The amount of products formed should remain the same OC. The amount of products formed should increase Clear my choice Mixing hydrogen peroxide with an enzyme different than catalase (such as lactase) should also result in the formation of products. Select one: O True O False The temperature at which an enzyme works best can differ from enzyme to enzyme. Select one: O True False B 7 OGiven the line-weaver Burke plot below for Enzyme Y, identify the Vmax for Enzyme Y. -10 30 1/v 1/[S] 5 10 Enter the numerical value for Vmax only, no letters or symbols. Round answer to 2 significant figures.Associate the biochemical reaction in TSI agar to certain genera or species. Reaction Possible Organism(s) K/K K/A K/A, H2S* A/A A/A A/A, H2S*
- The kinetics of this novel amylase were observed using lz in KI indicator to track the amount of starch at t-O min and t-1 min. They set up the following tubes and measured the absorbance after 1 minute of incubation. The reaction was stopped with HCl and 5 ml of Iz in KI indicator was added. One ml of each was used as sample in the spectrophotometer. Table 3. Calibration Curve Test tube Concentration of stock starch, mg/mL Volume of starch solution, ml Volume of distilled water, mL Volume of I, in Kl solution, mL Absorbance 1 10 0.0009 2 10 0.4 4.6 5 0.3306 3 10 0.8 4.2 5 0.5891 4 10 1.2 3.8 5 0.8409 15 10 1.6 3.4 1.0885 16 10 2.0 3.0 5 1.25728 7- 6- 2- 1- 45 90 135 180 225 270 315 360 405 450 495 540 58! [Substrate] (nM) What is the Vmax of the enzyme data shown above? rate (nM/sec) 3.An aerobic culture is grown in a continuous bioreactor at 28C and the product of yield was found to be 4 grams product produced per gram of oxygen consumed. It is planned to make this product at a rate of 6 kg/h. Calculate the overall heat of reaction per hour (in kJ/hr).
- A purified protein sample was used in a reaction, resulting in an activity of 696.7 nmol min-1. The reaction volume was 145.0 µL and the final volume before loading the plate was 1,050 µL. The total reaction time was 4.25 min. The amount of protein used in the reaction was 4.270 µg. Calculate the specific activity of the sample (in nmol min-1 µg-1).With a ∆G°´ of -16.7 kJ/mol, the reaction catalyzed by hexokinase is considered to be _____. at equilibrium substrate and product concentration dependent freely reversible metabolically irreversible none of the aboveProducts N ↓ N H N What effect would the enzyme have on the amount of product made in 3 seconds? Amounts Decrease No Change Increase N