sog a oue3 deauad s e un ao q sua au poue ongr) uau +9 upp o w np G+C Content asadal aag dw ds rs po sua u nosaorunda pe suruny eu s o pasn eu aqi 4 ps uor VNO 4 ir6padde auny VNG aedap pr unung anp oN ds aurs a u padnoul aq ue Pa ap para g od yN o sasshos a d por aay ogrgo apal o a d ae spo d dpon uaps uogexgp ao aued a doa f uep ao ao pasnq aq pos ogs p np as o oos aos rp anbs VN amp sposp a paoedau adn a ad iii tan aS jo suaqua ssop aa o aadap ap soU oipuqjo ua wi o VNa snodogouoy os aed-asn) r Phenotypic Methods t ng po pea os azpaqy VNG jo spunas alo the complementary strands of DNA from one organism will S11 aandy) samnap VNa a seao da aounqoqe LL p e VNG onos e q An osge p Sun 4 pousaap aq u s VNa popas-anop pr aunaad poog uad eply on o 4 pdo p a und 1-V seaaspao sadoplg an 4 alo p a und an 0 DNA Hybridization doo aaou ua uoq os poo p ae s300bas ous esqou ao sY 14101 ndy a0s) sds yaap jo sparsnoi uadj sp -dud paosoqu p 91 jo saanbos pse ouur soLndwoo so posng st dp a uy Aea os ao popondsa ead 1 paunduso3 aq ues spnpoad auad aac o 16 01 aundy ao) s ad rosoqu paaaP jo ur se rao pea oppe u VNC OSs o posn sanbeupo paepors a papoap ou susjurao 1u r ieoaddeo aespe uy ssupora o eqeses d prsosoqu snouRA JOsa0 p p VNG paps-aop ap p ad p lq panseu ugo 00L Aad up pynoo sas aouad poe saouebos apo pa a s ao a mp e uessa saop ao vosodaoo ang po foursV poa e M aes ng bu N Si0en 3.00 3.06 e the widespread availability of wbole and partial penoee joo s uoorngu YNG E 2ag u sa a sal n pa sos anesog s sg 3aads pagar psop undp o aaun uajo s y e upoa ssast s sg aubos VNG 0Ss lsogry o o saonbos yNQ Oss Sunureos sasnp pamd aq wag u sanbos au pasuehas pur poop ade pap Ngnss ap pue pr p ps sads pr aa woy poe aq es yNd au 092 1e 0xuequosqe ageja VT Selectiha an prcedure hh faster most diructly ffee free atimiaby Type aun ber population mples such as ed using PCR be compared ST ces of known mas been very tantly related ishing closely ed but geneti- al SSU rDNA s a better tool Os .06 00 artial genome NN Dd d o-agnop se sase0 u 09 denatures (melts) d amino acid s for determin- a op auanbos YNa jo so hat it accounts hniques used anism has an related. A similarity of base compositions, however, does not figure 10.9), atly expanded comparisons bosomal pro- igure 10.1b). termined, the necessarily mean that the organisms are related, because the nucleotide sequences and genome sizes could differ greatly. The GC content is often measured by determining the tem- perature at which the double-stranded DNA denatures, or melts. DNA that has a high GC content melts at a higher temperature because G-C base pairs are held together by three hydrogen bonds, whereas A-T pairs are held together by only two hydro- gen bonds. The temperature at which double-stranded DNA melts can be determined by measuring the absorbance of UV light by a solution of DNA as it is heated. The absorbance rap- idly increases as the DNA denatures (figure 10.15). ue UMI UEO