• the role of water as a medium for the metabolic processes of cells • the ability of water to moderate temperature within living organisms and in organisms' environment • the movement of water from the roots to the leaves of plants
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![(b) Explain each of the following in terms of the properties of water. You are not limited to the three properties discussed in part (a):
• the role of water as a medium for the metabolic processes of cells
• the ability of water to moderate temperature within living organisms and in organisms' environment
• the movement of water from the roots to the leaves of plants
B IU x2 X, 5 ¢
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- (b) Explain each of the following in terms of the properties of water. You are not limited to the three properties discussed in part (a): •the role of water as a medium for the metabolic processes of cells the ability of water to moderate temperature within living organisms and in organisms' environment • the movement of water from the roots to the leaves of plants B IUX² x, 3 ¢ 0/1 II(b) Explain each of the following in terms of the properties of water. You are not limited to the three properties discussed in part (a): • the role of water as a medium for the metabolic processes of cells • the ability of water to moderate temperature within living organisms and in organisms' environment • the movement of water from the roots to the leaves of plants B IU 0/1* Calculate the Molarity of solution have 20 g of Glucose sugar C12H22012 Dissolved in 125 g of water and the density of the solution Was 1.02 g/ml? If you know molar weight (C=12, O=16, H=1) O 0.412 M 0.401 M O 0.392 M
- Calculate the amount of rice bran (12.5% CP), corn (8.5% CP), soybean meal (49% CP) and copra meal (20% CP) in formulating 100 kg diet with 20% CP. Assume that the energy mixture will have a ratio of 2:1 and protein ingredients proportion is 1:3. Show your solution:Compare the solubility of lead carbonate in each of the following aqueous solutions: Clear All 0.10 M Pb(CH3C00)2 More soluble than in pure water. 0.10 M (NH4)2CO3 Similar solubility as in pure water. 0.10 Μ KNΟS Less soluble than in pure water. 0.10 Μ ΝΗCH, COOStoichiometric equations can be used to represent the growth of microorganisms provided a 'molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O, and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.301.2No.86. These bacterial cells are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is: 1 C16H34 + 16.28 O2 +1.42 NH3 → 1.65 C4.4H7.301.2No.86 + 8.74 CO2+ 13.11 H20 You are in charge of a small batch fermenter for growing the bacteria you need to produce 8.2 kg of cells for inoculation of a pilot-scale reactor. a) What minimum amount of hexadecane substrate (in kg) must be contained in your culture medium? Assume 100% conversion of hexadecane to cells. b) What must be the minimum concentration of hexadecane (in kg/m3) in the medium if the fermenter working volume is 5.8 cubic meters? c) What…
- MW of LA Volume of NaOH used (ml) x Normality of NaOH x % t.a. (expressed as lactic acid)= 1000 x100 Volume or weight of sample (g or ml) Molecular Weight of Lactic Acid (MW of LA) = 90 g/mol %3DC.. The formation of a hydrogen bonding dimer of N-methylacetamide has been studied as a model for hydrogen bonding in proteins. The thermodynamic parameters (at 298 K) measured for this process in water are shown below. 2 H3C-N H N-methylacetamide CH3 H3C HN CH3 HN CH3 AG° = 3.1 kcal mol-1 AH° 0.0 kcal mol-1 AS° -10 cal mol-1 K-1 H3C N-methylacetamide hydrogen-bonded dimer a) Provide a molecular level reasoning for the value of AH° and sign of ASO for this process. b) The conclusion below may be drawn from these data. Explain how his conclusion is reached. Hydrogen bonds are unlikely to be a strong stabilizing factor for a protein folding in water. c) Do you agree with this conclusion? What might be a limitation of this model for describing protein folding? d) It is well known that hydrogen bonds are prevalent in folded protein structures, so they appear to be important. So, if this conclusion is correct, what is an alternative possible role of hydrogen bonds in protein folding?You are asked to prepare HEPES buffered saline with the following constituents: НЕРES 10mM KCI 5mM NaCl 135mM CaCl2 1mM M9SO4 0.6mM Glucose 6mM You have previously made up 100 mM stock solutions of HEPES, KCI, CaCl2, and M9SO4 and a 1M stock of NaCl. The MW of glucose is 180.16 g/mol What volume of HEPES would you require to make up 400ml of buffer?
- Compare the solubility of lead carbonate in each of the following aqueous solutions: Clear All 0.10 M Pb(NO3)2 More soluble than in pure water. 0.10 M K,CO, Similar solubility as in pure water. 0.10 M NH,NO3 Less soluble than in pure water. 0.10 M NACH3C00You are supplied with the following: / Jy word voorsien van die volgende: NaCl (Mr= 58,443 g /mol) 2.5M Tris-Cl, pH 8 solution /oplossing (1 Litre) EDTA (Mr= 380,2g/mol) 10% sodium dodecyl sulphate solution / natriumdodecyl sulfaat oplossing Proteïnase K solution / oplossing (50 mg dissolved / opgelos in 1 ml ddH2O) You need a digestion buffer consisting of the following: / Jy moet 'n verteringsbuffer op maak wat uit die volgende bestaan: 15m M NaCl 75 mM Tris-Cl,pH 8 16 mM EDTA,pH 8 0.8% sodium sulphate / natrium dodecyl sulfaat 0,75 mg/ml proteïnase K How will you prepare 500 ml of the digestion buffer? Show all your steps and calculations. Remember to explain exactly how you will make it up. / Hoe sal jy 500 ml van die verteringsbuffer voorberei? Wys al jou stappe en berekeninge. Onthou om te verduidelik presies hoe jy dit sal op maak.Phosphate buffered saline (PBS) is a physiological buffer often used in biology experiments. Because the concentrations are very low and small amounts are difficult to weigh out, it is often prepared as a 10X stock solution meaning that it is prepared at 10 times the working concentration which is 1X. 1X PBS is made up of Na2HPO4 10 mM (MW 141.9 g.mol-1); KH2PO4 8 mM (MW 136.08 g.mol-1), NaCl 137 mM (MW 58.44 g.mol-1) and KCl 74.5 mM (MW 74.5 g.mol-1). You decide to prepare a 1 L 10X solution of PBS. What is the Molar (M) concentration of each of the components in you 10X stock. Write your answers in the table below. How much of each of the components should you weigh out to prepare 1L of the 10X stock solutions. Write your answers in the table below.