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(a) how this differs from its orientation in mitochondria. Given the orientation of the components of ATP synthase complex in diagram A, describe (b) cavity of the vesicle become the region of low proton concentration or the region of high proton con- centration? Explain your reasoning. Under light illumination to activate bacteriorhodopsin to drive ATP synthesis, does the inside In the presence of a proton gradient sustained by continuous light illumination, addition of (c) oligomycın had no effect on ATP synthesis, but addition of dinitrophenol (DNP) resulted in ATP hydrol- ysis. Explain.

(a) how this differs from its orientation in mitochondria. Given the orientation of the components of ATP synthase complex in diagram A, describe (b) cavity of the vesicle become the region of low proton concentration or the region of high proton con- centration? Explain your reasoning. Under light illumination to activate bacteriorhodopsin to drive ATP synthesis, does the inside In the presence of a proton gradient sustained by continuous light illumination, addition of (c) oligomycın had no effect on ATP synthesis, but addition of dinitrophenol (DNP) resulted in ATP hydrol- ysis. Explain.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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3 Diagram A below illustrates schematically a classic experiment designed to test the chemi- osmotic hypothesis. Phospholipid vesicles were made to contain beef heart mitochondrial ATP syn- thase and bacteriorhodopsin, a light driven proton pump isolated from Halobacterium holobium. Under light illumination, proton translocation by bacteriorhodopsin results in ATP synthesis when ADP and inorganic phosphate (Pi) are added to the suspension of vesicles. Diagram B shows a plot of the rate of ATP synthesis as a function of the proton gradient ApH. Bacteriorhodopsin in synthetic vesicle 100F A outside inside 5아 Fo F1 0.3 0.6 0.9 1.2 A pH (a) how this differs from its orientation in mitochondria. Given the orientation of the components of ATP synthase complex in diagram A, describe (b) cavity of the vesicle become the region of low proton concentration or the region of high proton con- centration? Explain your reasoning. Under light illumination to activate bacteriorhodopsin to drive ATP synthesis, does the inside (c) oligomycın had no effect on ATP synthesis, but addition of dinitrophenol (DNP) resulted in ATP hydrol- ysis. Explain. In the presence of a proton gradient sustained by continuous light illumination, addition of ATP synthesis rate (nmol- min'. mg) B
Transcribed Image Text:3 Diagram A below illustrates schematically a classic experiment designed to test the chemi- osmotic hypothesis. Phospholipid vesicles were made to contain beef heart mitochondrial ATP syn- thase and bacteriorhodopsin, a light driven proton pump isolated from Halobacterium holobium. Under light illumination, proton translocation by bacteriorhodopsin results in ATP synthesis when ADP and inorganic phosphate (Pi) are added to the suspension of vesicles. Diagram B shows a plot of the rate of ATP synthesis as a function of the proton gradient ApH. Bacteriorhodopsin in synthetic vesicle 100F A outside inside 5아 Fo F1 0.3 0.6 0.9 1.2 A pH (a) how this differs from its orientation in mitochondria. Given the orientation of the components of ATP synthase complex in diagram A, describe (b) cavity of the vesicle become the region of low proton concentration or the region of high proton con- centration? Explain your reasoning. Under light illumination to activate bacteriorhodopsin to drive ATP synthesis, does the inside (c) oligomycın had no effect on ATP synthesis, but addition of dinitrophenol (DNP) resulted in ATP hydrol- ysis. Explain. In the presence of a proton gradient sustained by continuous light illumination, addition of ATP synthesis rate (nmol- min'. mg) B
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