Table 1- Comparison of the effect of catechol concentration on the amount of product formed. Absorbance Potato extract after 30mins (2nd reading) (mL) 1 Tube # la blank 2a 3a 4a 1 1 1 dH₂O Catechol (mL) (mL) 7 5 3 1 0 2 4 6 Absorbance 0 mins 1st reading 0.00 0.060 0.033 0-052 Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1?. Time for reading: 3:21 -0.11 Q5) Tube la serves as a control, but why is this control needed? Absorbance: Time for reading: 3.36 Absorbance: 0.197 Time for reading: 3.37 Based on the data from Table 1 answer these questions: Q1) What is the name of the enzyme found in potato extract? Answer: Catechol Q2) What is the substrate? Answer: (10) Q3) Name of product of this enzyme catalyzed reaction? Answer: Absorbance: 0.152 Time for reading: 3:39 Absorbance: 0.66 Catechol Benzoquinone Subtract 1st from 2nd reading -0.0M 0.137 0.11.19 10.119 Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different at the 2nd absorbance reading? Explain why - hint you need to mention enzyme in your answer.
Table 1- Comparison of the effect of catechol concentration on the amount of product formed. Absorbance Potato extract after 30mins (2nd reading) (mL) 1 Tube # la blank 2a 3a 4a 1 1 1 dH₂O Catechol (mL) (mL) 7 5 3 1 0 2 4 6 Absorbance 0 mins 1st reading 0.00 0.060 0.033 0-052 Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1?. Time for reading: 3:21 -0.11 Q5) Tube la serves as a control, but why is this control needed? Absorbance: Time for reading: 3.36 Absorbance: 0.197 Time for reading: 3.37 Based on the data from Table 1 answer these questions: Q1) What is the name of the enzyme found in potato extract? Answer: Catechol Q2) What is the substrate? Answer: (10) Q3) Name of product of this enzyme catalyzed reaction? Answer: Absorbance: 0.152 Time for reading: 3:39 Absorbance: 0.66 Catechol Benzoquinone Subtract 1st from 2nd reading -0.0M 0.137 0.11.19 10.119 Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different at the 2nd absorbance reading? Explain why - hint you need to mention enzyme in your answer.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Answer Questions 5 and 6
Transcribed Image Text:Table 1 - Comparison of the effect of catechol concentration on the amount of product formed.
Absorbance
Potato
extract
Absorbance
0 mins
after 30mins (2nd reading)
(mL)
1st reading
1
Tube #
la
blank
2a
3a
4a
1
1
1
dH₂O Catechol
(mL) (mL)
7
5
3
1
0
2
4
6
0.00
0.060
0.033
0-05-2
Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1?
Time for reading: 3:21
-0.11
Absorbance:
Time for reading: 3.36
Q5) Tube la serves as a control, but why is this control needed?
Absorbance:
0.197
Time for reading: 3.37
Based on the data from Table 1 answer these questions:
Q1) What is the name of the enzyme found in potato extract? Answer:
catechol
Q2) What is the substrate? Answer:
THO
Q3) Name of product of this enzyme catalyzed reaction? Answer:
Absorbance: 0.152
Time for reading: 3:39
Absorbance: . 166
ness
Catechol
Benzoquinone
Subtract 1st
from 2nd
reading
-0.01
0-137
0.11.19
0.119
Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different at
the 2nd absorbance reading? Explain why - hint you need to mention enzyme in your answer.
Q7) What is the relationship between catechol concentration and amount of benzoquinone formed?
column's data do you use for this interpretation?
Which
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