Velocity, activity units/mg protein 51 3 2 0 5 15 20 Aspartate concentration, mM - 10 Control With With CTP With ATP CTP 25 Figure 2: Kinetics of ATCase in the presence of ATP and CTP (based on Gerhart and Pardee, 1962). The kinetics of the ATCase reaction were examined using increasing concentrations of aspartate, in the presence and absence of CTP and ATP as shown in Figure 2. a. What information can you obtain by looking at the shapes of the curves in this figure? b. What kinetic parameter(s) change in the presence of CTP? What parameter(s) do not change? What is the significance of these observations? c. Answer question 2b for ATP.

Biochemistry
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ISBN:9781319114671
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Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Velocity, activity units/mg
protein
31
5
4
3
~
0
5
15
20
Aspartate concentration, mM
10
- Control - With CTP With ATP
25
Figure 2: Kinetics of ATCase in the presence of ATP and
CTP (based on Gerhart and Pardee, 1962).
The kinetics of the ATCase reaction were examined using increasing concentrations of aspartate, in
the presence and absence of CTP and ATP as shown in Figure 2.
a. What information can you obtain by looking at the shapes of the curves in this figure?
b. What kinetic parameter(s) change in the presence of CTP? What parameter(s) do not change?
What is the significance of these observations?
c. Answer question 2b for ATP.
Transcribed Image Text:Velocity, activity units/mg protein 31 5 4 3 ~ 0 5 15 20 Aspartate concentration, mM 10 - Control - With CTP With ATP 25 Figure 2: Kinetics of ATCase in the presence of ATP and CTP (based on Gerhart and Pardee, 1962). The kinetics of the ATCase reaction were examined using increasing concentrations of aspartate, in the presence and absence of CTP and ATP as shown in Figure 2. a. What information can you obtain by looking at the shapes of the curves in this figure? b. What kinetic parameter(s) change in the presence of CTP? What parameter(s) do not change? What is the significance of these observations? c. Answer question 2b for ATP.
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