Carbonic anhydrase of erythrocytes (Mr 30,000) has one of the highest turnover numbers known. It catalyzes the reversiblehydration of CO₂.H₂O + CO₂2H₂CO3This is an important process in the transport of CO₂ from the tissues to the lungs. If 5 µg of pure carbonic anhydrase catalyzesthe hydration of 0.35 g of CO₂ in 1 min at 37 °C at Vmax, what is the turnover number (kcat) of carbonic anhydrase?Kcat2.0466 ×107Incorrectmin-1

The value of Kcat represents the catalytic efficiency of an enzyme. It is also known as the turnover…

Answered: Carbonic anhydrase of erythrocytes (M,…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The kinetic data in the following table were obtained for the reaction of carbon dioxide and water to produce bicarbonate and hydrogen ion catalyzed by carbonic anhydrase. H2O + CO2--> HCO3 + H+ Carbon Dioxide Concentration (mmol L-') 1.25 2.5 5.0 20.0 1/Velocity (M'sec) 36 x 10³ 20 x 10³ 12 x 10³ 6 x 10³ [H. DeVoe and G. B. Kistiakowsky, J. Am. Chem. Soc. 1961 83 (2), 274-280.] Use Excel (or similar) to generate a Line Weaver-Burke plot, then use the equation for a linear-fitted trend line to calculate the substrate affinity of CO2 for carbonic anhydrase. Answer in units of mM. Enter numerical values only, no letters or symbols. Round answer to 2 significant figures.
The various forms of vitamin K Function as cofactors for enzymes with a key role in blood coagulation O Function as cofactors in the blood coagulation pathway, and as chemical antioxidants Have different functions; one form is an enzyme cofactor, and another is a potent antioxidant O All serve as antioxidants
Studies of oxygen transport in pregnant mammals show that the O2-saturation curves of fetal and maternal blood are markedly different when measured under the same conditions. Fetal erythrocytes contain astructural variant of hemoglobin, HbF, consisting of two α and two γ subunits (α2 γ2 ), whereas maternal erythrocytes contain HbA (α2β2).(a) Which hemoglobin has a higher affinity for oxygen under physiological conditions, HbA or HbF? Explain.(b) What is the physiological significance of the different O2 affinities?(c) When all the BPG is carefully removed from samples of HbA and HbF, the measured O2 -saturation curves (and consequently the O2 affinities) are displaced to the left. However, HbA now has a greater affinity for oxygen than does HbF. When BPG is reintroduced, the O2 -saturation curves return to normal, as shown in the graph. What is the effect of BPG on the O2 affinity of hemoglobin? How can the above information be used to explain the different O2 affinities of fetal and…
What is the Vmax of an enzyme at 1 μM with -4G RT k = 6.2x 10¹2 s-1 (Ideal gas constant R = 8.314 J K-1 mol-1) cat 0.14 μM S-1 1.4 µM s-1 14 μM s-1 0.14 mM s-1 1.4 mM s-1 activation energy (AG) of 55000 J/mol at 298K?
7l.3.3
The following table summarizes the standard redox potential of some components ofelectron transport chain.Can cytochrome a and a3 be put in complex I whereas cytochrome b and c1 are put incomplex III? Explain.
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A researcher wants to look for glucaronic acid conjugated metabolites in sample of urine(glucaronic acid conjugation is a common method of metabolism for excretion.) Pronotated glucaronic acid conjugated metabolite ions often lose anhydroglucaronic acid(176 Da) during low energy CID. Which triple quadrupole experiment might be best for finding these conjugates?
1.12 At elevated altitudes, the body adapts to the reduced barometric pressure to extract sufficient oxy- gen to permit normal metabolic functions and do work. For example, at an altitude of 3,650 m (close to 12,000 feet above sea level) the barometric pressure drops to 485 mmHg. For an oxygen pressure drop in the lungs of 30 mmHg, determine the oxygen uptake rate for a respiration rate of 20 breaths per minute. Estimate the oxygen saturation in venous blood if the hematocrit rises to 0.60 and the partial pressure of oxygen blood is at a partial pressure equal to 98% of the alveolar level.
A 300g red squirrel in a flow through respirometry chamber consumed 0.27 l O2/hr.a. What is it’s metabolic rate in kcal/hr?a. What is its metabolic rate per kg body mass?
pls solve this with in 1 hour

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