The level of transcription of a gene is tested by creating deletions in the gene as shown in the illustration. These modified genes are tested for their level of transcription: • (++) normal transcription levels • (+) low transcription levels (+++) high transcription levels. Which deletion is in an enhancer involved in regulating the gene? Normal gene 1 2 3 4 ++ Deletion 1 3 4 |+++ Deletion 2 1 3 4 ++ Deletion 3 1 X 3 4 + Deletion 4 1 2 3 4 ++ 2] 2.

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The level of transcription of a gene is tested by creating deletions in the gene as shown in the illustration.

These modified genes are tested for their level of transcription:
- (++) normal transcription levels
- (+) low transcription levels
- (+++) high transcription levels

Which deletion is in an enhancer involved in regulating the gene?

### Illustration Explanation

1. **Normal Gene**: 
   - Segments: 1 (blue), 2 (red), 3 (orange), 4 (purple)
   - Transcription Level: ++ (normal)

2. **Deletion 1**:
   - Deleted Segment: 1 (blue)
   - Remaining Segments: 2, 3, 4
   - Transcription Level: +++ (high)

3. **Deletion 2**:
   - Deleted Segment: 2 (red)
   - Remaining Segments: 1, 3, 4
   - Transcription Level: ++ (normal)

4. **Deletion 3**:
   - Deleted Segment: 3 (orange)
   - Remaining Segments: 1, 2, 4
   - Transcription Level: + (low)

5. **Deletion 4**:
   - Deleted Segment: 4 (purple)
   - Remaining Segments: 1, 2, 3
   - Transcription Level: ++ (normal)

**Conclusion**: Deletion 3, which disrupts Segment 3, is likely affecting an enhancer involved in regulating the gene due to the observed low transcription levels.
Transcribed Image Text:The level of transcription of a gene is tested by creating deletions in the gene as shown in the illustration. These modified genes are tested for their level of transcription: - (++) normal transcription levels - (+) low transcription levels - (+++) high transcription levels Which deletion is in an enhancer involved in regulating the gene? ### Illustration Explanation 1. **Normal Gene**: - Segments: 1 (blue), 2 (red), 3 (orange), 4 (purple) - Transcription Level: ++ (normal) 2. **Deletion 1**: - Deleted Segment: 1 (blue) - Remaining Segments: 2, 3, 4 - Transcription Level: +++ (high) 3. **Deletion 2**: - Deleted Segment: 2 (red) - Remaining Segments: 1, 3, 4 - Transcription Level: ++ (normal) 4. **Deletion 3**: - Deleted Segment: 3 (orange) - Remaining Segments: 1, 2, 4 - Transcription Level: + (low) 5. **Deletion 4**: - Deleted Segment: 4 (purple) - Remaining Segments: 1, 2, 3 - Transcription Level: ++ (normal) **Conclusion**: Deletion 3, which disrupts Segment 3, is likely affecting an enhancer involved in regulating the gene due to the observed low transcription levels.
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