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The hypothesis for the experiment shown in the picture  is that the TamC3 and TamR3 cell lines function, and grow the absence of estrogen, receptor function. Reduced reliance on estrogen, receptor signaling alters mTOR pathway activity. If this hypothesis is true, then mediators of mTOR signaling (Akt or p70S6K) should have reduced levels of phosphorylation . A. Where would you look to see this? B. To what degree does the data in the picture support the hypothesis?

The hypothesis for the experiment shown in the picture  is that the TamC3 and TamR3 cell lines function, and grow the absence of estrogen, receptor function. Reduced reliance on estrogen, receptor signaling alters mTOR pathway activity. If this hypothesis is true, then mediators of mTOR signaling (Akt or p70S6K) should have reduced levels of phosphorylation . A. Where would you look to see this? B. To what degree does the data in the picture support the hypothesis?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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The hypothesis for the experiment shown in the picture  is that the TamC3 and TamR3 cell lines function, and grow the absence of estrogen, receptor function. Reduced reliance on estrogen, receptor signaling alters mTOR pathway activity. If this hypothesis is true, then mediators of mTOR signaling (Akt or p70S6K) should have reduced levels of phosphorylation . A. Where would you look to see this? B. To what degree does the data in the picture support the hypothesis?
The image shows a Western blot analysis comparing protein expression levels across three cell lines: MCF-7, TamC3, and TamR3. It visually represents the expression of specific proteins and their phosphorylated forms as follows: 1. **P-Akt**: - A band is present under each cell line (MCF-7, TamC3, TamR3), indicating the presence of phosphorylated Akt protein. 2. **Total Akt**: - A band is seen under each cell line for total Akt, indicating total protein presence irrespective of its phosphorylation state. 3. **P-p70S6K**: - Bands are faint or absent under each cell line, suggesting varying or low levels of phosphorylated p70S6K protein across the samples. 4. **Total p70S6K**: - Bands are present under each cell line, indicating the presence of the total p70S6K protein. 5. **Actin**: - Bands are consistent across all cell lines, serving as a loading control to ensure equal protein loading in each lane. This analysis helps in understanding the differential protein expression and post-translational modifications in these cell lines.
Transcribed Image Text:The image shows a Western blot analysis comparing protein expression levels across three cell lines: MCF-7, TamC3, and TamR3. It visually represents the expression of specific proteins and their phosphorylated forms as follows: 1. **P-Akt**: - A band is present under each cell line (MCF-7, TamC3, TamR3), indicating the presence of phosphorylated Akt protein. 2. **Total Akt**: - A band is seen under each cell line for total Akt, indicating total protein presence irrespective of its phosphorylation state. 3. **P-p70S6K**: - Bands are faint or absent under each cell line, suggesting varying or low levels of phosphorylated p70S6K protein across the samples. 4. **Total p70S6K**: - Bands are present under each cell line, indicating the presence of the total p70S6K protein. 5. **Actin**: - Bands are consistent across all cell lines, serving as a loading control to ensure equal protein loading in each lane. This analysis helps in understanding the differential protein expression and post-translational modifications in these cell lines.
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