In the study by Amano et al. the authors developed a collection of RNA sequences that showed optimal binding and specificity for Fibroblast Growth Factor 5 (FGF5). In the table below are some FGF5 binding data for the lead RNA aptamer sequences: Aptamer F5f1 F5f3 F5f1_56 F5f3_56 Kon (x 105 M-1 S-1) 3.3±0.3 1.7 +0.3 1.8 ± 0.3 1.36± 0.05 Koff (x 10-5 s-1) 20±6 10+2 2.1±0.3 12.5± 0.7 "The kinetic parameters are represented by the mean ± standard error from three independent measurements. Kd (nm) 0.7 +0.2 0.57 + 0.02 0.118 + 0.003 0.92 +0.04 Based upon this data, which aptamer would you consider to be the primary receptor for the FGF5 ligand? Explain your answer.

In the study by Amano et al. the authors developed a collection of RNA sequences that showed optimal binding and specificity for Fibroblast Growth Factor 5 (FGF5). In the table below are some FGF5 binding data for the lead RNA aptamer sequences: Aptamer F5f1 F5f3 F5f1_56 F5f3_56 Kon (x 105 M-1 S-1) 3.3±0.3 1.7 +0.3 1.8 ± 0.3 1.36± 0.05 Koff (x 10-5 s-1) 20±6 10+2 2.1±0.3 12.5± 0.7 "The kinetic parameters are represented by the mean ± standard error from three independent measurements. Kd (nm) 0.7 +0.2 0.57 + 0.02 0.118 + 0.003 0.92 +0.04 Based upon this data, which aptamer would you consider to be the primary receptor for the FGF5 ligand? Explain your answer.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

gamma:delta T-cell receptors are also generated by gene rearrangement. Some T cells express gamma:delta T-cell receptors rather than alpha:beta T-cell receptors. The organization of the a locus and the d locus helps to ensure that each T cell cannot express both types of T-cell receptors. The mechanism involved is that: The rearrangement of a T-cell receptor a gene deletes the d locus on that allele. The rearrangement of a T-cell receptor d gene deletes the a locus on that allele. The RAG recombinase enzymes are down-regulated immediately after the first T-cell receptor genes rearrange. The a:b T-cell receptor signals the T cell to delete the d locus. The g:d T-cell receptor signals the T cell to delete the a locus.
Is RNA silencing an environmentally-induced epigenetic change? Explain.
Various kinds of biochemical events may explain why some disease alleles are dominant?
68) When Sperry and colleagues ablated the dorsal RGCs, the remaining ventral RGCS projected to the dorsal tectum. Conversely, when they ablated the ventral RGCs, the remaining dorsal RGCS projected to the ventral tectum. of su Ephrins and Ephs are distributed in dorsoventral gradients in both structures. EphB1 is expressed in the tectum, in a low-dorsal to high-ventral gradient. Ephrin-B1 and ephrin- B2 are expressed in the retina in a high-dorsal to low-ventral gradient. What do these expression patterns suggest about the mechanism used for dorsoventral patterning of RGCS projecting to the tectum? (3-4 sentences Max.
How can Western blot analysis build in the finding of Encoding of Temporal Signals by the TGF-b Pathway and Implications for Embryonic Patterning??
Enhancer RPA Gene A Use the diagram above, which depicts a chromosomal region less than 500,000 bp, to determine the expression of each gene under the scenarios specified in the table. Regulatory promoters are indicated by "RP". Genes A, B, and C have intermediate expression when no regulatory proteins are bound. Complete the table by choosing high, intermediate, or low to describe the expression level of each gene under each specified scenario (i and ii). No regulatory proteins bound (i) A repressor binds to RPA and an activator binds to RPC (ii) A regulatory protein binds at each of the activator, insulator, and silencer. Expression of A? Intermediate 1. [Select] 4. [Select] Insulator RPB Expression of B? 2. Gene B Intermediate 5. [Select] [Select] Silencer RPC Expression of C? 3. Gene C Intermediate 6. [Select] [Select]
Wnt4 is expressed in a variety of embryonic tissues. You decide to study this gene during mouse development Part 1) In the mouse embryo at gastrulations are Wnts concentrated at the anterior or posterior region? Part 2) You decide to conduct a Wnt4 immunoblot (Western blot) using tissues from mouse embryos. What key reagent do you need to carry out this method? Part 3) Your immunoblot shows that Wnt4 is expressed in the embryonic ovary. What also need to be expressed in the ovary for Wnt4 to have an effect there? Part 4) Outline in three dot points how Wnt4 can induce gene expression in neighbouring cells. Part 5) a. What method you would use to show that Wnt4 gene is required for ovary development in the mouse? b. In three dot point, outline how this method is carried out.
do you have any clues? GAP protein helps hydrolyze the GTP to GDP in RAS protein. Argenine 789 residue in GAP protein interacts with GTP in RAS protein. However, Arg 789 is mutated to Alanine. which are true or false? ____We expect Ala789 GAP to have no consequence for cell growth or survival signaling. ____Arg789 stabilizes the T-loop of GAP in the outward (active) position, so we expect Ala789 GAP to have no kinase activity. ___Arg789 participates in the GTP hydrolysis reaction carried out by RAS, so we expect RAS to be catalytically inactive when partnered with Ala789 GAP. ____Arg789 binds in the major groove of the DNA double helix, so we expect Ala789 GAP to be inactive as a transcriptional regulator. __Arg789 interacts with RAS switch 1 and switch 2 regions, so we expect the RAS switch to fail with Ala789 GAP. ____The RAS switch would be locked in the OFF position by Ala789 GAP, so we expect no constitutive RAS signaling and hence no risk of this tumor metastasizing. ____The…
How did Covid-19 influence the rapid adoption of telemedicine.
How can one most effectively silence the myofilament gene unc-22 in C. elegans to produce the twitching phenotype? O A. Screen thousands of strains for a spontaneous unc-22 mutation O B. Soak worms in mutagenic solutions of EMS and screen the progeny OC. Inject antisense strands of unc-22 RNA into worm ovaries, which will be taken up by germ line cells and expressed in progeny O D. Inject a high dose of unc-22 MRNA into worm ovaries, which will be taken up by germ line cells and expressed in progeny O E. Culture worms with E. coli transformed with a plasmid that codes for a dsRNA unc-22 construct
Describe the mechanism of genomic imprinting of the Igf2 gene in mammals.
1. With reference to the figure, discuss the results of the Western Blotting experiments, what they tell you about the behaviour of the estrogen receptor, and how this relates to its known biological activity. 2. Propose what suitable controls should be included for this experiment and explain what you would expect to see as results from these controls in the Western Blot in the figure.