1. With reference to the figure, discuss the results of the Western Blotting experiments, what they tell you about the behaviour of the estrogen receptor, and how this relates to its known biological activity. 2. Propose what suitable controls should be included for this experiment and explain what you would expect to see as results from these controls in the Western Blot in the figure. This question relates to the biological activity of the estrogen receptor (ER). ER is anuclear receptor and positively regulates the expression the c-Myc gene, atranscription factor found at elevated levels in numerous cancers.To understand the effect of drugs on the biological behaviour of the estrogen receptorin cells, you undertake Western Blotting analysis of a cell line that expresses arecombinant human estrogen receptor. After culturing the cells, you then lyse the cellsand fractionate them, generating membrane, cytosolic and nuclear protein fractionsfrom the whole cell lysate. After running these fractions on SDS-PAGE gels, youtransfer to nitrocellulose membrane and probe with monoclonal antibody conjugatesfor the proteins ER and c-Myc.The results of your Western Blotting experiment without any treatment are provided inFigure 1.Primary antibodyCell fractionAnti-ERAnti-c-mycMembraneCytoplasmNucleus| |

Name: 1. With reference to the figure, discuss the results of the Western Bl
Uploaded: 2024-03-20T06:42:55.000Z
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Description: 1. With reference to the figure, discuss the results of the Western Blotting experiments, what they tell you about the behaviour of the estrogen receptor, and how this relates to its known biological activity. 2. Propose what suitable controls should