What is the benefit of a gene “knockout”? MARK ALL THAT APPLY. Group of answer choices It allows you to identify the function of a gene within an organism It is easily accomplished by using the Cas9 protein alone without guide RNA It helps you develop in vitro assays to study the function of a gene Can determine the level of expression of a gene within an organism The genotype can be easily observed from the survival and behavior of the knockout organisms

Q: What is a genomic library? O collection of sequences made of DNA fragments O collection of vectors…

A: The molecule that contains the genetic information necessary for an organism's growth and…

Q: Does one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?

A: A molecular process in which some discontinuous change to nucleotide sequences in their genomic DNA…

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: DNA sequence specificity of the CRISPR Cas9 complex is determined by: Group of answer choices None…

A: CRISPR is the kind of bacterial adaptive immunity. Bacteria binds viral DNA to it's genome to make…

Q: Which of the following statements about base editing is correct? 1. It involves nCas9. 2. The end…

A: Base editing  It is an alternative tool to HDR-mediated replacement. This facilitates editing of…

Q: What are the advantages of the yeast-two-hybrid system over other in vitro methods used for…

A: The yeast -two- hybrid system is the  In - Vivo identification technique of protein interaction in…

Q: Besides the great potential of gene editing, what are the biggest risks?  How do scientist test to…

A: Gene editing is a rapidly advancing field of genetic engineering that allows for precise…

Q: 1.True or false: In a bacterial species with a "lac operon", the repressor protein will be bound to…

A: Prokaryotic gene regulatory system is known as operon system. In this process the expression of…

Q: 5. A promoter is a sequence of choose one: (amino acids / bases / DNA/RNA / 5-carbon sugars).…

A: Promoter sequence is a region of DNA upstream of a gene where proteins will bind to initiate…

Q: vnat Is the physiologic function of the CRISPR/Cas9 system? O It is used by bacteria to recognize…

A: CRISPR or "clustered regularly interspaced short palindromic repeats" are short viral origin DNA…

Q: What does CRISPR-Cas stand for and how does it work? What are the molecules involved with CRISPR-Cas

A: Introduction Genome editing:- It is also known as gene editing or genome engineering. It is a type…

Q: What is the Guide RNA (gRNA) a chimera of? Why use a gRNA?

A: Disclaimer: "According to the Bartleby guidelines, only the first question is to be answered first…

Q: fill in the blank: 1. The expression of a gene, its pre-mRNA can undergo   , resulting in multiple…

A: “Since you have posted a question with multiple sub-parts, we will solve first three sub-parts for…

Q: 24. The diagram below shows the map for EcoRI restriction sites around the BRCA1 gene for two…

A: Answer well explained above.

Q: 1- To generate a sequencing library of transcriptome, the cellular    is converted to a…

A: Hello. Since you have posted multiple questions and not specified which question needs to be solved,…

Q: LO65 Compare methods that Covid tests and Covid vaccines use.

A: Comparison of methods that Covid tests and Covid vaccines use. there are several test  method used…

Q: a researcher isolates mRNA from a healthy and diseased liver biopsies. each mRNA sample is converted…

A: RNA molecules were relegated as a simple intermediate between genes and proteins, as encapsulated in…

Q: What is it about the Cas9 protein that makes it an efficient DNAediting tool?

A: DNA and RNA are the genetic elements present in almost all cells. DNA encodes for proteins through…

Q: Below is shown an 8kb region of the human genome, with the proportion of the nucleotides that are…

A: Introduction :  Any segment of a gene that will be included in the mature RNA produced by that gene…

Q: You have identified a mutation. In the normal organism the DNA TEMPLATE strand is 5'AGT3' in the…

A: Mutations are changes in the sequence of DNA which may or may not affect the phenotype of the…

Q: a. CRISPR-Cas9 is a valuable biotechnology tool. Explain how this system uses RNA and the result of…

A: CRISPR-Cas9  is known as unique biotechnology tool which allows medical researchers and geneticists…

Q: Using CRISPR/Cas9 to target a gene in the mouse will primarily result in: a. Any of the choices b.…

A: CRISPR/Cas9 is a powerful tool that can be used to target and edit genes. This technology has a wide…

Q: It is true in RNAsrq technology ans microsrray..... both assess gene expression in protein…

A: Gene expression is the phenomena by which a gene gets activated in a cell to make RNA and proteins.…

Q: The figure below is a portion of a Northern blot which shows the change in abundance of the HS-X…

A: Introduction  In molecular biology, a Southern blot is a technique for identifying a specific DNA…

Q: What is the role of the following molecules in the CRISPR mechanism? *

A: CRISPR stands for clustered regularly interspaced short palindromic repeats. These are a set of DNA…

Q: For gene expression profiling, what does a microarray or “gene chip” measure directly? Group of…

A: Microassay is technology-based upon the fact that a complementary sequence of DNA can be used to…

Q: A mulitple cloning site (MSC) is used in TOPA-TA cloning in TA cloning with restriction enzymes both…

A: The process by which genetic material from one organism is introduced into another organism to…

Q: A transgene is successfully integrated into the genome of an animal zygote. The transgene is under…

A: The foreign DNA (gene) is incorporated into a host organism by the process of recombinant DNA…

Q: Which one of the following statements is FALSE regarding the Ames test? liver enzymes are added to…

A: Ames test is a test used to determine genotoxicity via a chemical mutagen .  It generally work on…

Q: What is the most accurate way to introduce mutations to particular regions in the genome? Using…

A: An modification to the genome's nucleotide sequence of an organism, virus, extrachromosomal DNA, or…

Q: An EMS mutagenesis screen identified several mutants in this gene. The figure shows the position of…

A: "As per the Q&A guidelines, we will provide the solution to the first question. Please repost…

Q: 10) Scientists have engineered a simplified CRISPR-cas system for use in mammalian cells. What were…

A: The clustered regularly interspaced short palindromic repeats (CRISPR) is a technology that is used…

Q: 14) Do the cDNA libraries generated from a mouse brain and a mouse liver contain ALL of the same DNA…

A: A eukaryotic gene consists of coding (exons) as well as non-coding (introns) regions. During the…