Your insert has the sequence TGGATCCTTATAATGTTT.....Which pET-41 vector will you use to clone with BamHI and to keep insert in frame? a b C Ncol BamHI pET-41a(+): ATG...gst...CC/C ATG GGA TAT CGG G/GA TCC GAA TTC Met Gly Tyr Arg Gly Ser Glu Phe Met Pro pET-41b(+): ATG...gst...CC/C Met ATG GAT ATC GGG/ GAT CCG AAT TC Pro Met Asp lle Gly Asp Pro Asn

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Need help with understanding how to set up, pick and find reading frame for doing a gene insert...

Your insert has the sequence
TGGATCCTTATAATGTTT.....Which pET-41
vector will you use to clone with BamHI and to keep insert in frame?
a
b
C
Ncol
BamHI
PET-41a(+): ATG...gst...CC/C ATG GGA TAT CGG G/GA TCC GAA TTC
Pro Met Gly Tyr Arg Gly Ser Glu Phe
Met
Question
PET-41b(+): ATG...gst...CC/C ATG GAT ATC GGG/GAT CCG AAT TC
Pro Met Asp lle Gly Asp Pro Asn
Met
PET-41c(+): ATG...gst...CC/C ATG GCG ATA TCG GG/G ATC CGA ATT C
Pro Met Ala lle Ser Gly lle Arg lle
Met
Transcribed Image Text:Your insert has the sequence TGGATCCTTATAATGTTT.....Which pET-41 vector will you use to clone with BamHI and to keep insert in frame? a b C Ncol BamHI PET-41a(+): ATG...gst...CC/C ATG GGA TAT CGG G/GA TCC GAA TTC Pro Met Gly Tyr Arg Gly Ser Glu Phe Met Question PET-41b(+): ATG...gst...CC/C ATG GAT ATC GGG/GAT CCG AAT TC Pro Met Asp lle Gly Asp Pro Asn Met PET-41c(+): ATG...gst...CC/C ATG GCG ATA TCG GG/G ATC CGA ATT C Pro Met Ala lle Ser Gly lle Arg lle Met
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