Your insert has the sequence CTGAATTCTATAATGCCC.....Which pET-41 vector will you use to clone with EcoRI and keep insert in frame? a b C Ncol BamHI EcoRI pET-41a(+): ATG...gst...CC/C ATG GGA TAT CGG G/GA TCC G/AA TTC Pro Met Gly Tyr Arg Gly Ser Glu Phe Met pET-41b(+): ATG...gst...CC/C ATG GAT ATC GGG/ GAT CCG/AAT TC Met Asp lle Gly Asp Pro Asn Met Pro pET-41c(+): ATG...gst...CC/C ATG GCG ATA TCG GG/G ATC CG/A ATT C lle Arg lle Pro Met Ala lle Ser Gly Met

Q: What is one major way in which next generation sequencing differs from dideoxy sequencing (Sanger)?

A: The technique of sequencing DNA involves identifying the nucleic acid sequence, or the arrangement…

Q: How do we use genomic or cDNA libraries to find our gene of interest in a forward genetic screen?

A: Reverse genetics:It is an approach in molecular genetics. It is used to help in understanding the…

Q: Discuss how CRISPR-cas functions as a genome editing tool.

A: Genome editing:It is also known as gene editing or genome engineering. It is a type of genetic…

Q: What is exome sequencing used for?

A: DNA sequencing:   It is a method to find a sequence of nucleotides by using the DNA sequencing…

Q: Describe the methods of site-directed mutagenesis and CRISPR-Cas technology.

A: A molecular process in which some discontinuous change to nucleotide sequences in their genomic DNA…

Q: Besides the great potential of gene editing, what are the biggest risks?  How do scientist test to…

A: Gene editing is a rapidly advancing field of genetic engineering that allows for precise…

Q: How is ribotyping similar to rRNA sequence analysis? How do the two techniques differ? Do you think…

A: RFLP is a method to check genetic markers in the genome of organisms. The genetic markers are…

Q: Discuss the importance of introducing the CPT coding system to healthcare

A: CPT is current procedural terminology. It consist of medical code set and each code describes…

Q: Do not use chatgpt.

A: Goals of human genome project:Obtain a physical map of genome- genome; this allows the rough…

Q: Within genomics, genetic engineering and gene therapy are two topics that are quite comparable. What…

A: Genomics is the study of an organism's entire genetic complement (the genome). It sequences,…

Q: Outline the goals and major findings of the ENCODE Project.

A: ENCODE stands for "Encyclopedia of DNA Elements". It was a research project that came after the…

Q: Describe the basic components of CRISPR.

A: CRISPR mechanism is useful for the bacteria in protecting themselves from the attack of viruses. It…

Q: 23. Describe each type of transposon and how they integrate into the DNA. How are transposons…

A: Genome is defined as the complete set of genetic information that make up all the chromosomes of the…

Q: Besides gene expression, what else can be assayedusing gene chips?

A: DNA microassays or DNA chips contain thousands of DNA sequences of individuals arrayed at a great…

Q: Describe how transposable elements are used as experimental tools.

A: Mutation is defined as sudden inheritable change that occurs in the DNA sequence. Transposon also…

Q: What types of cells are used for cloning rDNA?

A: rDNA is called recombinant DNA which contains DNA from multiple sources. Recombinant DNA is it's the…

Q: What is the difference between using genome build hg18 and hg19 in Genome-wide SNP arrays?

A: SNP (Single nucleotide protein) array is a type of DNA (deoxyribonucleic acid) microarray which is…

Q: Explain why accuracy is more important for DNA replication than for transcription

A: DNA replication is that the method by that a double-stranded DNA molecule is traced to provide 2…

Q: Outline the key steps involved in cloning Dolly the sheep.

A: Cloning is a process used to create genetic copies of an organism .This technique came into action…

Q: describe the main steps required to clone a gene and produce a protein.

A: Gene cloning is the technique of locating and copying (cloning) a gene of interest from all the DNA…

Q: What does whole exome sequencing reveal?

A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…

Q: RNAi is a method to assess........ gene expression    gene sequence  gene function  Chromosomal…

A: A gene is a functioning heredity unit made up of DNA, which provides instructions for the creation…

Q: How can we use the transposon sequence to find where the mutation occurred in a forward genetic…

A: Forward genetic screes, also termed as forwarding genetics, is a kind of process by which those…

Q: Discuss some potential issues regarding gene editing to correct genetic defects.

A: In conclusion, the use of Genetics can prove helpful but should only ever be done after weighing all…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.