The KAS1 gene has been identified as a target for and experimental CRISPR-based therapy for Non-Small Cell Lung Cancer. Four potential sgRNAs have been designed (see figure 1). + strand - strand CDS sgRNA 1 2 3 4 KAS1 Offtargets identified 0,0,3,11,207 0,0,0,17,207 0,0,0,17,205 0,0,1,13,232 Figure 1 - four sgRNAs have been proposed, numbered 1 - 4.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Which sgRNA would you consider for further testing and why? In answer consider the location of the target and the relevance of “off targets”. Include an explanation of series of numbers relating to “off targets identified”
The KAS1 gene has been identified as a target for and experimental CRISPR-based therapy
for Non-Small Cell Lung Cancer. Four potential sgRNAs have been designed (see figure 1).
+ strand
- strand
CDS
sgRNA
1
2
3
4
KAS1
Offtargets identified
0,0,3,11,207
0,0,0,17,207
0,0,0,17,205
0,0,1,13,232
Figure 1 - four sgRNAs have been proposed, numbered 1 - 4.
Transcribed Image Text:The KAS1 gene has been identified as a target for and experimental CRISPR-based therapy for Non-Small Cell Lung Cancer. Four potential sgRNAs have been designed (see figure 1). + strand - strand CDS sgRNA 1 2 3 4 KAS1 Offtargets identified 0,0,3,11,207 0,0,0,17,207 0,0,0,17,205 0,0,1,13,232 Figure 1 - four sgRNAs have been proposed, numbered 1 - 4.
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