12. You want to determine the location of the promoter and operator for an operon. To do so, you create the following deletions and assay their phenotype. The thin lines indicate the deleted region. expression d1 inducible inducible d2 d3 constitutive d4 none d5 none d6 inducible Which of the following can you conclude from this data? a. Part of the promoter is within deletion 1 b. Part of the operator is within deletion 3 c. The promoter and the operator are very close to each other d. Both b and c e. Both a and b

12. You want to determine the location of the promoter and operator for an operon. To do so, you create the following deletions and assay their phenotype. The thin lines indicate the deleted region. expression d1 inducible inducible d2 d3 constitutive d4 none d5 none d6 inducible Which of the following can you conclude from this data? a. Part of the promoter is within deletion 1 b. Part of the operator is within deletion 3 c. The promoter and the operator are very close to each other d. Both b and c e. Both a and b

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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The organization and function of the lac operon in E. coli is shown in the following figures: XXI Transcription Repressor mRNA Translation RNA polymerase M M Flask ZYA XXXX Active M-repressor protein Repressor active, operon off. The repressor protein binds with the operator, preventing transcription from the operon. (a) An inducible operon Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Allolactose (inducer) Carbon/Energy Source 0 Z MInactive repressor protein (a) An inducible operon Transcription Translation XXX Permease B-Galactosidase Transacetylase Operon mRNA 3 Repressor inactive, operon on. When the inducer allolactose binds to the repressor protein, the inactivated repressor can no longer block transcription. The structural genes are transcribed, ultimately resulting in the production of the enzymes needed for lactose catabolism. Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings Suppose you inoculate three flasks of minimal…
2. Assume you have identified a new operon in bacteria (which you call the suc operon) that encodes enzymes required for catabolism of the sugar sucrose. The structural genes that are required to breakdown sucrose are transcribed from a promoter (Psuc). One of these enzymes is encoded by the sucS gene. Expression of the operon is controlled by a regulator, called SucR, which binds to an operator (O). Using your knowledge of the lac operon, interpret the results of the following experiments (Table 1) to propose a model describing how the sucrose operon might be regulated. Cell genotype Production of SucS enzyme Without sucrose With sucrose R* O* P* s* R O* p* s* ++ R* O P* S* -- RO* p* s* / R* Oʻ p* 5 R° O* p* S ++ -- ++ ++ Table 1. Production of SucS enzyme in various strains. “-" in the cell genotype indicates a loss of function mutation, “+" indicates wild type function, two genotypes separated by a / indicate a merodiploid or partial diploid. R° is a mutant version of SucR that…
Operons A. Draw a schematic of the lac operon when glucose is low and lactose is high in the cell’s environment. B. Draw a schematic of the trp operon when tryptophan levels in the environment are low. C. What is the lux operon and how is affected by quorum sensing?
Explain how the lac operon is regulated under the following conditions. Include the following terms in your answers: Repressor, Operator, RNA polymerase, CAP site, CAP protein, Promoter A. Lactose and glucose are both present. B. Lactose and glucose are both absent. C. Lactose is present and glucose is absent.
47.
A sample of E.coli cells is transformed with a plasmid containing a transcriptional fusion including the promoter of an E.coli housekeeping gene and the LacZ gene. The transformed cells are plated out on selective medium containing X-gal. Which of the following best describes the likely results. a. Colonies will only be blue if lactose is also included in the growth medium. b. Colonies will only be blue if lactose and glucose are included in the growth medium c. Most of the colonies should turn blue. d. Presence of any white colonies may indicate deletion or mutation of the promoter. e. Both c. and d. are correct
Please Explain
3 5. Suppose you performed a B-galactosidase assay on E. coli with a l*P+O°Z*Y operon. What results would you expect in the absence and presence of IPTG? 6. If you did not get the expected results for the previous question, but you knew the genotype of the operon was correct, name two ways your experiment could have gone wrong.
1. Suppose there was a mutation in the inducer of the lac operon, such that the inducer was unable to function. How would the lac operon be affected? Please explain your reasoning in 1-3 sentences.
1. Please tell me: What's a mutation in moleculer terms? 2. When a mutation deletes a base in genomic DNA, how does it affect the expression product production and reading frame? 3. How does induction control enzyme synthesis using lactose operon?
In the tryptophan operon, if the leader peptide is translated normally A region 2 will pair with region 3 B a rho independent terminator will form. C both a and b D none of the above
28. Which statement does NOT explain why the lac operon would not be effectively regulated by an attenuation mechanism like the trp operon? 1.Lactose is a substrate for the lac operon protein products. 2.Products of lac operon enzymes are not used in protein synthesis. 3.Transcription and translation do not happen simultaneously for the lac operon. 4.Attenuation is a suitable regulatory mechanism for anabolic amino acid pathways. 5.All of these statements explain why regulation by attenuation would not be effective for the lac operon.