Table 18.2 Characteristics of some common type II restriction enzymes used in recombinant DNA technology Microorganism from Type of fragment Enzyme which enzyme is isolated Recognition sequence end produced BamHI Bacillus amyloliquefaciens 5-GGATCC-3 Cohesive 3'-CCTAGG-3' EcoRI Escherichia coli 5'-GAATTC-3 Cohesive 3'-CTTAAG-5' EcoRII Escherichia coli 5-CCAGG-3' 3'-GGTCC-5' Cohesive Haelll Haemophilus aegyptius 5-GGCC-3' 3-CCGG-5 Blunt HindIII Haemophilus influenzae 5'-AAGCTT-3' 3'-TTCGAA-5 Cohesive Notl Nocardia otitidis-caviarum 5-GCGGCCGC-3 Cohesive 3'-CGCCGGCG-5 Pstl Providencia stuartii 5-CTGCAG-3' Cohesive 3'-GACGTC-5' ↑ Smal Serratia marcescens 5-CCCGGG-3' Blunt 3'-GGGCCC-5'
Table 18.2 Characteristics of some common type II restriction enzymes used in recombinant DNA technology Microorganism from Type of fragment Enzyme which enzyme is isolated Recognition sequence end produced BamHI Bacillus amyloliquefaciens 5-GGATCC-3 Cohesive 3'-CCTAGG-3' EcoRI Escherichia coli 5'-GAATTC-3 Cohesive 3'-CTTAAG-5' EcoRII Escherichia coli 5-CCAGG-3' 3'-GGTCC-5' Cohesive Haelll Haemophilus aegyptius 5-GGCC-3' 3-CCGG-5 Blunt HindIII Haemophilus influenzae 5'-AAGCTT-3' 3'-TTCGAA-5 Cohesive Notl Nocardia otitidis-caviarum 5-GCGGCCGC-3 Cohesive 3'-CGCCGGCG-5 Pstl Providencia stuartii 5-CTGCAG-3' Cohesive 3'-GACGTC-5' ↑ Smal Serratia marcescens 5-CCCGGG-3' Blunt 3'-GGGCCC-5'
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The following is the partial sequence of a bacterial gene ORF: 5’ --------------------CGGAATTCCCGGGGATCC------------------------3’ (The remaining sequence of the gene ORF never affects the cloning of this gene. The multiple cloning sites of the plasmid vector can be cleaved by 8 restriction endonucleases that are listed in the table.
5’- and 3’-end sequences of the bacterial gene ORF are 5’ ATGGAGT TATCCAGGTGCCT--- and 3’AATATGGAGTTATCCAGGTGCCT---, respectively. After choosing the appropriate restriction endonuclease(s) for directional cloning using the table below, determine the sequences of two 20-mer primers used for the PCR amplification of the gene ORF.
Transcribed Image Text:Table 18.2 Characteristics of some common type II restriction enzymes used in recombinant
DNA technology
Microorganism from
Type of fragment
Enzyme
which enzyme is isolated
Recognition sequence
end produced
BamHI
Bacillus amyloliquefaciens
5-GGATCC-3
Cohesive
3'-CCTAGG-3'
EcoRI
Escherichia coli
5'-GAATTC-3
Cohesive
3'-CTTAAG-5'
EcoRII
Escherichia coli
5-CCAGG-3'
3'-GGTCC-5'
Cohesive
Haelll
Haemophilus aegyptius
5-GGCC-3'
3-CCGG-5
Blunt
HindIII
Haemophilus influenzae
5'-AAGCTT-3'
3'-TTCGAA-5
Cohesive
Notl
Nocardia otitidis-caviarum
5-GCGGCCGC-3
Cohesive
3'-CGCCGGCG-5
Pstl
Providencia stuartii
5-CTGCAG-3'
Cohesive
3'-GACGTC-5'
↑
Smal
Serratia marcescens
5-CCCGGG-3'
Blunt
3'-GGGCCC-5'
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