You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured the bacteria and gone through the plate isolation and purification procedure. After culturing the pure sample strain, what is the most appropriate order of the next several procedures (ignoring DNA concentration and A260 ratio checks)? Genomic DNA Extraction --> Amplification of Targeted Gene --> Purification of PCR product --> Visualization via Gel Electrophoresis --> Sequencing Amplification of Targeted Gene --> Visualization via Gel Electrophoresis --> Genomic DNA Extraction --> Sequencing Genomic DNA Extraction --> Amplification of Targeted Gene--> Visualization via Gel Electrophoresis --> Purification of PCR product Sequencing None of these are the most appropriate order to follow These can be done in any order, so all of the answers are correct
You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured the bacteria and gone through the plate isolation and purification procedure. After culturing the pure sample strain, what is the most appropriate order of the next several procedures (ignoring DNA concentration and A260 ratio checks)? Genomic DNA Extraction --> Amplification of Targeted Gene --> Purification of PCR product --> Visualization via Gel Electrophoresis --> Sequencing Amplification of Targeted Gene --> Visualization via Gel Electrophoresis --> Genomic DNA Extraction --> Sequencing Genomic DNA Extraction --> Amplification of Targeted Gene--> Visualization via Gel Electrophoresis --> Purification of PCR product Sequencing None of these are the most appropriate order to follow These can be done in any order, so all of the answers are correct
Microbiology for Surgical Technologists (MindTap Course List)
2nd Edition
ISBN:9781111306663
Author:Margaret Rodriguez, Paul Price
Publisher:Margaret Rodriguez, Paul Price
Chapter4: The Prokaryotes
Section: Chapter Questions
Problem 4UTM
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Transcribed Image Text:You will use the following scenario to answer a group of 5 questions. You have isolated a
microbe from an environmental sample. The microbe has the ability to perform a new
metabolic reaction at a very low temperature, so you are excited that it could be a new
species.
You have already cultured the bacteria and gone through the plate isolation and purification
procedure. After culturing the pure sample strain, what is the most appropriate order of the
next several procedures (ignoring DNA concentration and A260 ratio checks)?
Genomic DNA Extraction --> Amplification of Targeted Gene --> Purification of PCR product -->
Visualization via Gel Electrophoresis --> Sequencing
Amplification of Targeted Gene --> Visualization via Gel Electrophoresis --> Genomic DNA Extraction
--> Sequencing
Genomic DNA Extraction --> Amplification of Targeted Gene--> Visualization via Gel Electrophoresis
--> Purification of PCR product Sequencing
None of these are the most appropriate order to follow
These can be done in any order, so all of the answers are correct
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