You will use the following scenario to answer a group of 5 questions. You have isolated amicrobe from an environmental sample. The microbe has the ability to perform a newmetabolic reaction at a very low temperature, so you are excited that it could be a newspecies.You have already cultured the bacteria and gone through the plate isolation and purificationprocedure. After culturing the pure sample strain, what is the most appropriate order of thenext several procedures (ignoring DNA concentration and A260 ratio checks)?Genomic DNA Extraction --> Amplification of Targeted Gene --> Purification of PCR product -->Visualization via Gel Electrophoresis --> SequencingAmplification of Targeted Gene --> Visualization via Gel Electrophoresis --> Genomic DNA Extraction--> SequencingGenomic DNA Extraction --> Amplification of Targeted Gene--> Visualization via Gel Electrophoresis--> Purification of PCR product SequencingNone of these are the most appropriate order to followThese can be done in any order, so all of the answers are correct

Solution:The correct sequence in order is: Genomic DNA Extraction → Amplification of Targeted Gene →…

Answered: You will use the following scenario to…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000
The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?
There are so many microbes in a single mL of culture, it is very difficult to perform one dilution to produce countable cells. Microbiologists need to perform a dilution series, where multiple dilutions are performed in sequence to arrive at the correct dilution. Dilutions are cumulative. Multiple the series of dilutions together to find the final dilution value. If 3 serial dilutions are performed, each with a value of 0.01, what is the cumulative dilution? Express your answer as an exponent, e.g. 0.1 would be 1e-1 and 0.01 would be 1e-2
You are tasked to observe the motility of an UNKNOWN Bacteria isolated from a mixed culture, which technique/s is BEST to perform? How shall the procedure be carried out? Explain.
You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs. You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution scheme
A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using I ml aliquot in 9 ml of 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. 田 Sampling COUNTS 1st dilution 54; 61 2nd dilution 3rd dilution 4h dilution 1st 3; 7 0; 0 0,0 2nd TNTC TNCT 242: 233 28: 37 3rd TNTC TNTC INTC 249-246 * TNTC = Too numerous to count i. Illustrate the dilution series used and label the final dilution of each dilution. ii. Determine the bacterial count (CFU/ml) every 4 hours of incubation for 12 hours. Show all computations.
You are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method what other methods could you use to separate the bacteria? Please state what method(s) you would use, the result you would be looking for to help identify each bacterium and the interpretation of the result.
You are tasked with quantifying bacteria in your soil sample. You resuspend 1.0g of soil in 10 ml 1x PBS, then do a 10-fold serial dilution of your soil sample. You plate 150 µl onto each plate. The 104 dilution plate contains 175 colonies after 2 days of incubation, how many bacteria do you estimate were present in your initial soil sample? (# colonies/volume plated in ul) * dilution factor * ul convert to ml * volume suspended soil (ml/g) = Read the formatting instructions below before entering your answer! 1. Make sure your answer is in correct scientific notation using an "e" to indicate the exponent! For example, 2500 would be 2.5e3, 0.0066 would be 6.6e-3. 2. For answers greater than 1, round up to the nearest tenth. For example, 2515 would be 2.5e3 and 51510 would be 5.2e4.
A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/L
1. Sally Monella found 344 bacterial colonies on one of her plates. She had prepared this plate after a serial dilution of a culture of Yersinia pestis. She had pipetted 1 ml sample of diluted Yersinia pestis from a tube with the overall dilution factor of 106 into a plate and covered it with agar. She used this plate to calculate the concentration in cells/ml of a bacterial culture. a) How many organisms were in the original culture? b) Were her results valid? Why or why not?
you are given a mix culture of S aureus, E. coli and P. aeruginosa. besides using the streak plate method what other method could you choose to separate the bacteria? the result you will be looking for and interprete the result.
You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?

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