Lucy examines the graph she made after analysing her experiment, which has the shape shown below. Isoleucine uptake (mmol/L cells/min) Time (min) At the point at which the curve has levelled off: Select one: a. The isoleucine uniporter has become saturated with substrate b. Isoleucine has equilibrated across the cell membrane, and its rate of influx via the isoleucine uniporter is the same as its rate of efflux C. The isoleucine uniporter has equilibrated isoleucine across the cell membrane, and switches off (stops functioning) after having done so ○ d. All of the radiolabelled isoleucine added to the cells is now inside the cells, so no more can be transported in e. All of the potential explanations above are equally likely

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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Lucy is studying isoleucine transport by a neutral amino acid uniporter in her cell type of interest. She determines that the initial rate of radiolabelled isoleucine uptake in 0.3 mL of her cell suspension was 78 cpm/min. In her experiment, 5% of the volume of the cell suspension was made up by the cells themselves.

Lucy examines the graph she made after analysing her experiment, which has the shape
shown below.
Isoleucine uptake
(mmol/L cells/min)
Time (min)
At the point at which the curve has levelled off:
Select one:
a. The isoleucine uniporter has become saturated with substrate
b. Isoleucine has equilibrated across the cell membrane, and its rate of influx via
the isoleucine uniporter is the same as its rate of efflux
C. The isoleucine uniporter has equilibrated isoleucine across the cell membrane,
and switches off (stops functioning) after having done so
○ d. All of the radiolabelled isoleucine added to the cells is now inside the cells, so no
more can be transported in
e.
All of the potential explanations above are equally likely
Transcribed Image Text:Lucy examines the graph she made after analysing her experiment, which has the shape shown below. Isoleucine uptake (mmol/L cells/min) Time (min) At the point at which the curve has levelled off: Select one: a. The isoleucine uniporter has become saturated with substrate b. Isoleucine has equilibrated across the cell membrane, and its rate of influx via the isoleucine uniporter is the same as its rate of efflux C. The isoleucine uniporter has equilibrated isoleucine across the cell membrane, and switches off (stops functioning) after having done so ○ d. All of the radiolabelled isoleucine added to the cells is now inside the cells, so no more can be transported in e. All of the potential explanations above are equally likely
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