17. What type of microscopic image is this? Why would you use this type of microscopy? How does it stain the sample? R. FETTER (2/27/04) 18. If I want to look at the surface of a plant what type of microscopy would I need to use and why?
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- PROCEDURE: 1. Set two pencils down parallel from each other. Make them about 2-3 inches apart as the length of your slides to keep things easy. 2. Stick a long piece of tape over the two pencils and to the table on either side of the pencils to hold the tape tightly between the two pencils like a bridge. 3. Don’t touch the sticky side of the tape or you will ruin the microscope. Drop a small drop of water onto the top of the tape using the pipette or medicine dropper. 4. Make 3-4 lines of tape and add a different-sized drop to each one. This will help determine what size of water droplet produces the biggest magnification. 5. Prepare a rectangular shape of plastic cover. Put a small slice of onion. Slide the rectangular shape of the plastic cover with the small slice of onion under the pieces of tape and observe the size of the onion on different droplets. Write your observations on the table below. No. of drops ObservationWhat are the advantages and disadvantages of using the Wet Mount technique? What are the advantages and disadvantages of using the Hanging drop technique? What are the advantages and disadvantages of using the Slide Culture technique? pls elaborate each, thank youThe specimen you are examining is very thin and transparent. What can you do to make it easier to see
- Select all that apply to a direct simple stain: 1. the dye is acidic or negatively charged and stains the background 2. uses multiple dyes in the procedure 3. cells will never be distorted or shrunken 4. uses only 1 dye in the procedure 5. does involve a washing step 6. can be used to determine cell morphology, size, and arrangement. 7. does involve fixing 8. the dye is basic or positively charged and stains the bacteria 9. cells may be distorted or shrunken 10. does not involve a washing step 11. does not involve fixingFirst step : Below I have attached the image of gram stain. -We need to find the name of the compound. And after that , what possible test can be performed to find the compound. We need to do 6 test . So can you please help me with the names for the test to be performed to find the compound. Thank you so much ?Microtome 1. Define microtome instrument 2. Mention the types of microtome instrument 3. What are the principle operation of any type of microtome? 4. Compare between two typo of microtome difference from principle operation
- Which part of the microscope is used to adjust the light? (pick one) i. h. a. d. c.The significance of using immersion oil when using 1OOX objective lens is The significance of using immersion oll when uskng 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized. the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce i. il. iii. iv. A light microscope should have the following features contrast Resolution magnification fluorescence ii. iv.Select all that apply to a negative stain: 1. involves a washing step 2. cells may be distorted or shrunken 3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cells