Homework Help is Here – Start Your Trial Now!
Science
Biology
R Amp 8,000 Xhol 0 (or 12,000) PUC 18 12,000 bp 2,000 insert 4,000 EcoRI alone HindIll alone Xhol alone EcoRI+ Hindlll EcoRI + Xhol HindIll + Xhol 12,000 bp 12,000 bp 12,000 bp 3,000 and 9,000 bp 2,000 and 10,000 bp 5,000 and 7,000 bp You have cloned a DNA fragment into the plasmid pUC18 shown above. The pUC vector (solid part) contains the ampicillin (AmpR) resistance gene, as well as one recognition site for the restriction enzyme Xhol and one recognition site for EcoRI, but no recognition sites for Hindill. The inserted DNA fragment is indicated by the striped bar. You perform several restriction digests of the recombinant plasmid, using each enzyme alone or in combination with another enzyme. The sizes of the resulting DNA fragments are shown in the table 30) At what position number on the plasmid is the HindIll site? a) 2,000 bp b) 3,000 bp c) 6,000 bp d) 8,000 bp

R Amp 8,000 Xhol 0 (or 12,000) PUC 18 12,000 bp 2,000 insert 4,000 EcoRI alone HindIll alone Xhol alone EcoRI+ Hindlll EcoRI + Xhol HindIll + Xhol 12,000 bp 12,000 bp 12,000 bp 3,000 and 9,000 bp 2,000 and 10,000 bp 5,000 and 7,000 bp You have cloned a DNA fragment into the plasmid pUC18 shown above. The pUC vector (solid part) contains the ampicillin (AmpR) resistance gene, as well as one recognition site for the restriction enzyme Xhol and one recognition site for EcoRI, but no recognition sites for Hindill. The inserted DNA fragment is indicated by the striped bar. You perform several restriction digests of the recombinant plasmid, using each enzyme alone or in combination with another enzyme. The sizes of the resulting DNA fragments are shown in the table 30) At what position number on the plasmid is the HindIll site? a) 2,000 bp b) 3,000 bp c) 6,000 bp d) 8,000 bp

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
100%
Amp 8,000 Xhol 0 (or 12,000) I PUC 18 12,000 bp 2,000 insert 4,000 EcoRI alone HindIll alone Xhol alone EcoRI + Hindlll EcoRI + Xhol HindIll+ Xhol 12,000 bp 12,000 bp 12,000 bp 3,000 and 9,000 bp 2,000 and 10,000 bp 5,000 and 7,000 bp You have cloned a DNA fragment into the plasmid pUC18 shown above. The pUC vector (solid part) contains the ampicillin (AmpR) resistance gene, as well as one recognition site for the restriction enzyme Xhol and one recognition site for EcoRI, but no recognition sites for Hindill. The inserted DNA fragment is indicated by the striped bar. You perform several restriction digests of the recombinant plasmid, using each enzyme alone or in combination with another enzyme. The sizes of the resulting DNA fragments are shown in the table + 30) At what position number on the plasmid is the HindIll site? a) 2,000 bp b) 3,000 bp c) 6,000 bp d) 8,000 bp
Transcribed Image Text:Amp 8,000 Xhol 0 (or 12,000) I PUC 18 12,000 bp 2,000 insert 4,000 EcoRI alone HindIll alone Xhol alone EcoRI + Hindlll EcoRI + Xhol HindIll+ Xhol 12,000 bp 12,000 bp 12,000 bp 3,000 and 9,000 bp 2,000 and 10,000 bp 5,000 and 7,000 bp You have cloned a DNA fragment into the plasmid pUC18 shown above. The pUC vector (solid part) contains the ampicillin (AmpR) resistance gene, as well as one recognition site for the restriction enzyme Xhol and one recognition site for EcoRI, but no recognition sites for Hindill. The inserted DNA fragment is indicated by the striped bar. You perform several restriction digests of the recombinant plasmid, using each enzyme alone or in combination with another enzyme. The sizes of the resulting DNA fragments are shown in the table + 30) At what position number on the plasmid is the HindIll site? a) 2,000 bp b) 3,000 bp c) 6,000 bp d) 8,000 bp
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 4 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Nutrition & Digestion
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS