EcoRI alone HindIll alone Bam HI alone EcoRI+HindIll EcoRI+BamHI HindIll+ BamHI 15,000 bp 15,000 bp 15,000 bp 7,000 and 8,000 bp 4,000 and 11,000 bp 4,000 and 11,000 bp Amp PUKY plasmid 15,000 bp 2,000 EcoRI insert 7,000 You have cloned a DNA fragment into the plasmid pUKY shown above. The insert contains one EcoRI site but no recognition sites for Hind!!! or BamHI. The position of the EcoRI site is indicated on the figure below. The extent of the inserted DNA fragment is indicated by the striped bar. You perform several restriction digests of the recombinant DNA, using each enzyme alone or in combination with another enzyme. The sizes of the multing roctriction fragments are shown in the table.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Answer :- In molecular biology, restriction enzymes play a pivotal role in the analysis and manipulation of DNA. They are proteins that can cleave DNA at specific recognition sequences, producing fragments of varying sizes.
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