1. You have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions. a. Pscl & Gsul b. Scal, Pdml & BsaXI c. Scal, Sspl & Ehel

Q: digest suspect B's DNA using restriction enzyme 1. Restriction enzyme 1 cleaves the recognition site…

A: The restriction enzymes are endonucleases that digest DNA (deoxyribonucleic acid) molecules by…

Q: What is the principle of the Restriction Fragment Length Polymorphism (RFLP) in the diagnosis of…

A: Restriction fragment length polymorphism (RFLP) : it is a method used in genome mapping and…

Q: ased on the knowledge you gained from the cloning module, which of the lanes in the urs is…

A: Agarose gel electrophoresis is used to separate DNA molecules based on their size and analyze DNA…

Q: 83. A plasmid is cut with the restriction enzyme BamH1 giving fragments of 3000 and 1000 bp as…

A: In this plasmid, we got 2 fragments of length 1000 and 3000 bp after digestion with BamH1 that…

Q: The other options are:   a. RNA cannot be digested by restriction enzymes b. RNA is small enough…

A: Northern blotting involve separation of RNA fragments by gel electrophoresis. In contrast Southern…

Q: A PCR reaction was performed to amplify the CHE2 gene. The PCR primers used were designed to amplify…

A: Polymerase chain reaction is a molecular biology technique that is sued to synthesize multiple…

Q: Match the term with its description.

A: There are various enzymes and gene components that are studied in techniques of genetic engineering.…

Q: Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the…

A: The type of restriction enzymes that cut double stranded DNA at a particular spot is known as…

Q: 5. You wish to map restriction sites in an unknown plasmid that has not been sequenced. You digest…

A: The process of fragmenting deoxyribonucleic acid strands with specific enzymes is restriction…

Q: 18. An instructor had her students perform this laboratory beginning with setting up their own…

A: Agarose gel electrophoresis is the technique for the separation of DNA fragments on the basis of…

Q: This plasmid is digested with the enzymes listed below. Indicate how many fragments will be…

A:

Q: Why is the presence of insertion sequences on the F plasmid significant? What are F- prime (or F')…

A: The process of fusion of two compatible bacterial cells, is known as Conjugation. It requires the…

Q: Please answer the following using the experiment data below. Provide the formulas or methods used…

A: Transformation efficiency is defined as the number of colonies obtained per microgram of DNA plated.

Q: 5 μL of plasmid DNA (pUC 19) was added to 50ul of CaCl2 competent E. coli that was then added 250 μL…

A: Introduction :- A plasmid is a small, circular, double-stranded DNA molecule that can replicate…

Q: arac PKAN-R 5,512 bp PARA 4,872 bp BamH PBAD Bam 377 bp kank ampR PBAD-rfp 807 bp Hindill Hindli

A: Answer :  The ristricton enzmye used to make cut in the plasmid.  Both the plasmid should be cut…

Q: 35) A researcher inserted DNA fragments from an organism into expression vector plasmids and…

A: A plasmid is a small, circular, extra double stranded molecule of DNA that is being different from…

Q: Write the 3' to 5' DNA bottom strand • Write the mRNA sequence of top strand Write the mRNA sequence…

A: In DNA   there are four nitrogen as base that is adenine, Thymine, guanine and cytosine is present.…

Q: You are cloning a gene from a eukaryotic organism into a plasmid to produce a recombinant protein in…

A: The biological process by which naked DNA (deoxyribonucleic acid) gets transferred from one…

Q: 4. Animation 12.4: This table shows results from a genetic test conducted on DNA samples from two…

A: Restriction enzymes are the enzymes capable of cutting double-helical segments that contain a…

Q: Draw a diagram showing what pGEM will look like after it has been digested with BamHI.

A: BamHI: BamHI is a restriction enzyme obtained from Bacillus amyloliquefaciens that specifically cuts…

Q: 27. Given the following plasmid map, list how many fragments would exist and their bp length when…

A: Restriction enzymes are used by bacteria to cleave foreign DNA. They can then be isolated and used…

Q: 2. A plasmid was digested with the enzymes BamHI, ECORI, and HindIII. A single digest was performed…

A: A restriction map describes the location of all the restriction sites on a DNA (deoxyribonucleic…

Q: A student wanted to load .75 ug dna on agarose gel and has 4x loading buffer for sample preparation.…

A: Agarose gel electrophoresis is a method used for the separation of DNA fragments which is stained by…

Q: When using the plasmid shown below for cloning, which restriction enzyme site(s) (all indicated with…

A: A plasmid is a double-stranded DNA molecule that is small and circular in shape.  Bactria and yeast…

Q: Which of the following would allow for control of a gene's orientation when inserted into a plasmid…

A: The technique of molecular biology used to generate similar copies of a particular segment (gene) of…

Q: Select all the following statements that are correct regarding agarose gel electrophoresis and…

A: Agarose gel electrophoresis is a process used for separation of DNA fragments. Steps of agarose gel…

Q: F plasmid F’ plasmid (and how is it formed) Hfr cell (and how is it formed)

A: Plasmids are the extrachromosomal material that exist apart from the main chromosome in a bacterial…

Q: X ↓ O 650 O 550 O 500 200 O 850 Smal ✓ O 1550 300 Msel 350 Probe Smal ↓ Caption: Restriction enzyme…

A: Restriction endonuclease are called molecular scissors because they cut at specialised DNA…

Q: 2. Use the plasmid to answer the following questions: [6T] a) Digest the plasmid with EcoRI only and…

A: The restriction enzymes are used to make nicks at certain positions on the DNA. These positions are…

Q: Briefly explain why the total size of the pMBBS plasmid in the Restriction Enzymes practical is…

A: Plasmid:Circular mostlySize of plasmid is between <5kb - >200kbMay or may not be present in…

Q: 14) Which antibiotic plasmid has been used? (kanamycin, ampicillin or tetracycline?) 15) Which of…

A: 14  )   tetracycline is the antibiotic plamid.

Q: Uncut - DNA Fragments produced B2 Sspl Enzyme(s) EcoR1 Smal used - 5.0 plasmid, how many times does…

A: Introduction :  The DNA-based structure known as a plasmid can exist and replicate on its own in…

Q: You cut a plasmid with a restriction enzyme that cuts it into three pieces-one fragment is 280 base…

A: Plasmids are circular double stranded DNA. These are basically found in the unicellular organisms.…

Q: Renal failure will almost always result in the development of bone disorders. Briefly describe the…

A: Introduction :  Renal Osteodystrophy is the term used to describe the overall skeleton abnormalities…

Q: Restriction maps illustrate the lengths of DNA fragments between restriction sites. Which of the…

A: Restriction mapping is a technique to map unknown segment of DNA by breakung it into small…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.