27. Given the following plasmid map, list how many fragments would exist and their bp length when treated with Hindll, and Nde1

Q: 51. In 1973, Cohen and Boyer created the first chimera by successfully incorporating the frog rRNA…

A: In 1973, Boyer and Cohen successfully recombined the two plasmids pSC 101 that contain gene…

Q: Do virtual restriction digests of 300ng pGLO plasmid using HinDIII, EcoRI, and XhoI separately, and…

A: DNA restriction enzymes cleaves the DNA at specific sites. These recognition sites have DNA…

Q: 83. A plasmid is cut with the restriction enzyme BamH1 giving fragments of 3000 and 1000 bp as…

A: In this plasmid, we got 2 fragments of length 1000 and 3000 bp after digestion with BamH1 that…

Q: You start with a relaxed DNA plasmid that is 1000bp in length. a). What is the starting linking…

A: Since you have posted a question with multiple sub-parts, we will solve the first sub-part for you.…

Q: Using the plasmid map attached and the answer from the previous question, draw a simplified diagram…

A: The image you provided shows a diagram of the YCplac33 plasmid. This plasmid is a commonly used…

Q: 5. You wish to map restriction sites in an unknown plasmid that has not been sequenced. You digest…

A: The process of fragmenting deoxyribonucleic acid strands with specific enzymes is restriction…

Q: This plasmid is digested with the enzymes listed below. Indicate how many fragments will be…

A:

Q: 1. Under each sequence of bases, write the sequence of bases complementary to each section of DNA in…

A: Introduction :  The primary nucleobases are cytosine (DNA and RNA), guanine (DNA and RNA), adenine…

Q: arac PKAN-R 5,512 bp PARA 4,872 bp BamH PBAD Bam 377 bp kank ampR PBAD-rfp 807 bp Hindill Hindli

A: Answer :  The ristricton enzmye used to make cut in the plasmid.  Both the plasmid should be cut…

Q: During plasmid extraction, after the addition of acetate-conta in ing neutralization buffer the…

A: Plasmids are extrachromosomal DNA in the bacterial cells. They are double stranded circular DNA,…

Q: How can you tell if you have had a successful induced transformation using: "C" = control, "T"…

A: Transformation refers to the process of introducing foreign DNA (such as plasmid DNA) into bacterial…

Q: 25. The restriction enzymes Kpnl and Acc65l recognize and cleave the same 6-bp sequence. You have a…

A: Restriction enzymes are the DNA digesting enzymes.

Q: Briefly explain why the total size of the pMBBS plasmid in the Restriction Enzymes practical is…

A: Plasmid:Circular mostlySize of plasmid is between <5kb - >200kbMay or may not be present in…

Q: 1a.The complete genotype of the host yeast strain is MATa ade2 his3 leu2 met15 ura3.  Name one…

A: Hi! Thanks for your question. As the second part of the question (b) is incomplete because it does…

Q: Following formation of a double-stranded break, enzymes process the dsDNA end to form…

A: DNA end resection is a key process in the cellular response to DNA double-strand break damage that…

Q: Question: You conclude that all the blue colonies contain a plasmid

A: pUC is a plasmid cloning vector which is created by Joachim Messing and co-workers. Restriction…

Q: is shown below. Draw a restriction map of a plasmid with the appropriate restriction sites based on…

A: Restriction digestion is the fragmentation(cutting) of DNA on very specific sites (restriction…

Q: You are studying a group of individuals with X-ray vision and perform linkage analysis and note that…

A: DNA cloning is the procedure in genetic engineering used for the production of a larger number of…

Q: In an restriction enzyme experiment where Eco RI and  Hind III are used . What would happen if a…

A: Introduction  An isolated bacterial protein known as a restriction enzyme cleaves DNA at…

Q: A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid…

A: Plasmids are small, circular, double stranded, and extrachromosomal bacterial DNA. i.e. it is…

Q: 28. In a transformation experiment, DNA is collected from an E. coli donor strain of genotype cys(+)…

A: There are three modes of transfer of genetic information in bacteria. They are transduction,…

Q: This strand is 18% A, 12% T, 36% G, and 34% C.

A: Chargaff's rules: As you mentioned, the key lies in remembering Chargaff's rules. Adenine (A) always…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.