1.1kbp 1.5 kbp Hindll Hindll 0.15 kbp amp 0.25 kbp If you were to digest this plasmid with HindllI, how many fragments would be visible using gel electrophoresis? U23 4.
1.1kbp 1.5 kbp Hindll Hindll 0.15 kbp amp 0.25 kbp If you were to digest this plasmid with HindllI, how many fragments would be visible using gel electrophoresis? U23 4.
Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Question
Transcribed Image Text:### Plasmid Digestion and Gel Electrophoresis
#### Plasmid Map Analysis
The diagram depicts a circular plasmid map with specific restriction enzyme recognition sites and corresponding fragment sizes. Key points include:
1. **EcoRI Sites**: There are two EcoRI recognition sites on this plasmid.
- One site is located between the fragments 1.1 kbp and 1.5 kbp.
- Another EcoRI site is next to the fragment labeled 0.25 kbp.
2. **HindIII Sites**: There are two HindIII recognition sites on this plasmid.
- The exact positions are not labeled relative to the entire plasmid size but are near the fragment labeled 0.15 kbp.
3. **Labeled Fragment Sizes**:
- 1.1 kbp
- 1.5 kbp
- 0.15 kbp
- 0.25 kbp (adjacent to the amp marker, which likely denotes an ampicillin resistance gene)
#### Question for Understanding
"If you were to digest this plasmid with HindIII, how many fragments would be visible using gel electrophoresis?"
##### Possible Answers:
- 1
- 2
- 3
- 4
- 5
### Detailed Explanation
When analyzing the result of a plasmid digestion:
- **HindIII Digestion**: Digestion with HindIII cuts at the two HindIII sites shown on the plasmid.
Given there are two HindIII sites on the plasmid, digesting with HindIII will produce two fragments because it will cut the circular plasmid at these two locations, linearizing it.
### Quiz Answer
With HindIII digestion, the correct answer is:
- **2** fragments.
#### Interactive Learning
- Students are encouraged to predict the result of an EcoRI digestion based on the positions of the recognition sites and compare them with HindIII digestion results.
- Experiment with different restriction enzyme combinations to explore the relationship between cutting sites and fragment sizes.
This exercise aims to strengthen understanding of restriction digestion and gel electrophoresis in a molecular biology context.
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