You are cloning the genome of a new DNA virus into pUC18. You plate out your transformants on ampicillin plates containing X-gal and pick one blue colony and one white colony. When you check the size of the inserts in each plasmid (blue and white), you are surprised to find that the plasmid from the blue colony contains a very small insert of approximately 60 bp, while the plasmid from the white colony does not appear to contain any insert at all. Explain these results.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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2AQ:You are cloning the genome of a new DNA virus into pUC18.
You plate out your transformants on ampicillin plates containing X-gal and pick one blue
colony and one white colony. When you check the size of the inserts in each plasmid (blue
and white), you are surprised to find that the plasmid from the blue colony contains a very
small insert of approximately 60 bp, while the plasmid from the white colony does not
appear to contain any insert at all. Explain these results.

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