1. You make a recombinant plasmid vector that contains an insert of a gene you are interested in studying (see below on left). After making your recombinant vector you run it on an agarose gel to check it (See below on right). You run some of the uncut vector and see 3 bands (Uncut lane). You also cut some recombinant vector with both restriction enzymes BamHI and HindIII and run this on the gel too (Cut lane). Agarose Gel Recombinant Plasmid vector with insert -ve BamHI insert Hindi- bp 5,000 2,300 2,000 1,800 1,000 500 300 DNA Marker Uncut A B Cut D +ve
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Q. What is the total size of your recombinant plasmid vector containing the insert?
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