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DNA ladder is attached (the picture shows HindIII as H, EcoRI as E, BamHI as B). Now, determine the size of fragments created by EcoRI and BamHI. In the graph attached there is the fragment size of HindIII. HindIII EcoRI BamHI Migration Distance (mm) Fragment Size (bp) Migration Distance (mm) Fragment Size (bp) Migration Distance (mm) Fragment Size (bp)   23455           11985           7430           4375           2150           1850

DNA ladder is attached (the picture shows HindIII as H, EcoRI as E, BamHI as B). Now, determine the size of fragments created by EcoRI and BamHI. In the graph attached there is the fragment size of HindIII. HindIII EcoRI BamHI Migration Distance (mm) Fragment Size (bp) Migration Distance (mm) Fragment Size (bp) Migration Distance (mm) Fragment Size (bp)   23455           11985           7430           4375           2150           1850

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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 DNA ladder is attached (the picture shows HindIII as H, EcoRI as E, BamHI as B). Now, determine the size of fragments created by EcoRI and BamHI. In the graph attached there is the fragment size of HindIII.

HindIII

EcoRI

BamHI

Migration Distance (mm)

Fragment Size (bp)

Migration Distance (mm)

Fragment Size (bp)

Migration Distance (mm)

Fragment Size (bp)

 

23455

 

 

 

 

 

11985

 

 

 

 

 

7430

 

 

 

 

 

4375

 

 

 

 

 

2150

 

 

 

 

 

1850

 

 

 

 

 

 

This image displays the results of a DNA electrophoresis gel, commonly used in molecular biology for separating DNA fragments based on size. The gel shows several lanes labeled H, B, and E. ### Detailed Description: - **Lanes**: - **H**: Contains multiple distinct bands, indicating various DNA fragment sizes. - **B**: Displays bands at different positions than those in lane H, suggesting different fragment sizes or molecular weights. - **E**: Shows a distinct pattern of bands compared to both H and B, with varying intensities, which represent different quantities or concentrations of DNA fragments. - **Arrow**: - Located at the top left, indicating the direction of DNA migration towards the positive electrode. ### Gel Electrophoresis Explanation: - **Principle**: DNA fragments are separated by size as they migrate through a gel matrix under an electric field. Smaller fragments move faster and travel further than larger ones. - **Interpretation**: Each lane represents a different sample or enzyme digest, with bands corresponding to DNA fragments. The pattern and number of bands can be analyzed to determine the physical characteristics of the DNA samples. This type of visualization is crucial for genetic analysis, identification of gene fragments, or verification of DNA cloning.
Transcribed Image Text:This image displays the results of a DNA electrophoresis gel, commonly used in molecular biology for separating DNA fragments based on size. The gel shows several lanes labeled H, B, and E. ### Detailed Description: - **Lanes**: - **H**: Contains multiple distinct bands, indicating various DNA fragment sizes. - **B**: Displays bands at different positions than those in lane H, suggesting different fragment sizes or molecular weights. - **E**: Shows a distinct pattern of bands compared to both H and B, with varying intensities, which represent different quantities or concentrations of DNA fragments. - **Arrow**: - Located at the top left, indicating the direction of DNA migration towards the positive electrode. ### Gel Electrophoresis Explanation: - **Principle**: DNA fragments are separated by size as they migrate through a gel matrix under an electric field. Smaller fragments move faster and travel further than larger ones. - **Interpretation**: Each lane represents a different sample or enzyme digest, with bands corresponding to DNA fragments. The pattern and number of bands can be analyzed to determine the physical characteristics of the DNA samples. This type of visualization is crucial for genetic analysis, identification of gene fragments, or verification of DNA cloning.
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