Q3.2- In the Anfinsen protein folding experiment, removing urea and BME at the same time led to functional refolding of the protein. Explain why removing BME without removing urea leads to a nonfunctional enzyme. Why does adding small amounts of BME alone to the solution with the nonfunctional enzyme "rescue" the function? !
Q3.2- In the Anfinsen protein folding experiment, removing urea and BME at the same time led to functional refolding of the protein. Explain why removing BME without removing urea leads to a nonfunctional enzyme. Why does adding small amounts of BME alone to the solution with the nonfunctional enzyme "rescue" the function? !
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:Mechanisms of Protein Folding
Q3.2- In the Anfinsen protein folding experiment, removing urea and BME
at the same time led to functional refolding of the protein. Explain why
removing BME without removing urea leads to a nonfunctional enzyme.
Why does adding small amounts of BME alone to the solution with the
nonfunctional enzyme "rescue" the function?
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