10. Explain the difference at the molecular level in how temperature and urea denature a protein.
10. Explain the difference at the molecular level in how temperature and urea denature a protein.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
10
![10:16 AM Fri Sep 2
<
то
8. Predict the effect of each of the following environmental changes on the pKa of a glutamate side
chain and explain your logic.
The N-terminus of the protein is brought into the proximity of glutamic acid
A lysine side chain is brought into the proximity of glutamic acid
the glutamic acid side chain is shifted from the outside of the protein to the interior
9. A small protein was completely hydrolyzed and found to contain (in no particular order):
Arg, Cys, Lys, Met, Phe, Thr,
a. Incubation with FDNB generated Sanger Reagent yielded DNP-Lys
b. Incubation with chymotrypsin had no effect on the peptide
c. Incubation with trypsin yielded free arginine and lysine
d. Incubation with cyanobromide yielded a tetrapeptide capable of disulfide bonding.
10. Explain the difference at the molecular level in how temperature and urea denature a protein.
11. Based on the denaturation curve shown here for two separate proteins, A and B:
a. Label the y-axis:
b. Which protein is more stable? Why?
c. Other than urea concentration, what is another
potential parameter for the x-axis?
b) Chaperone Proteins:
c) Protein Domain:
1,0
d) Protein Data Bank:
0.8-
0.6
0.4-
0.2
12. Define the following (note: you my need to use internet resources to do so):
a) Native state:
0.0
@83%
+:
Protein A
A
Protein B
Urea concentration (M)
< WIN >](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa545a84d-531c-4165-a2c5-d8f9043a6753%2Fc3fca402-06b7-4593-b620-3f05c0c9b073%2Fpomad7l_processed.jpeg&w=3840&q=75)
Transcribed Image Text:10:16 AM Fri Sep 2
<
то
8. Predict the effect of each of the following environmental changes on the pKa of a glutamate side
chain and explain your logic.
The N-terminus of the protein is brought into the proximity of glutamic acid
A lysine side chain is brought into the proximity of glutamic acid
the glutamic acid side chain is shifted from the outside of the protein to the interior
9. A small protein was completely hydrolyzed and found to contain (in no particular order):
Arg, Cys, Lys, Met, Phe, Thr,
a. Incubation with FDNB generated Sanger Reagent yielded DNP-Lys
b. Incubation with chymotrypsin had no effect on the peptide
c. Incubation with trypsin yielded free arginine and lysine
d. Incubation with cyanobromide yielded a tetrapeptide capable of disulfide bonding.
10. Explain the difference at the molecular level in how temperature and urea denature a protein.
11. Based on the denaturation curve shown here for two separate proteins, A and B:
a. Label the y-axis:
b. Which protein is more stable? Why?
c. Other than urea concentration, what is another
potential parameter for the x-axis?
b) Chaperone Proteins:
c) Protein Domain:
1,0
d) Protein Data Bank:
0.8-
0.6
0.4-
0.2
12. Define the following (note: you my need to use internet resources to do so):
a) Native state:
0.0
@83%
+:
Protein A
A
Protein B
Urea concentration (M)
< WIN >
Expert Solution
![](/static/compass_v2/shared-icons/check-mark.png)
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
Recommended textbooks for you
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781319114671/9781319114671_smallCoverImage.jpg)
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
![Lehninger Principles of Biochemistry](https://www.bartleby.com/isbn_cover_images/9781464126116/9781464126116_smallCoverImage.gif)
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
![Fundamentals of Biochemistry: Life at the Molecul…](https://www.bartleby.com/isbn_cover_images/9781118918401/9781118918401_smallCoverImage.gif)
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781319114671/9781319114671_smallCoverImage.jpg)
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
![Lehninger Principles of Biochemistry](https://www.bartleby.com/isbn_cover_images/9781464126116/9781464126116_smallCoverImage.gif)
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
![Fundamentals of Biochemistry: Life at the Molecul…](https://www.bartleby.com/isbn_cover_images/9781118918401/9781118918401_smallCoverImage.gif)
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781305961135/9781305961135_smallCoverImage.gif)
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781305577206/9781305577206_smallCoverImage.gif)
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
![Fundamentals of General, Organic, and Biological …](https://www.bartleby.com/isbn_cover_images/9780134015187/9780134015187_smallCoverImage.gif)
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON