Q.3 Following data were obtained for an enzyme that catalyzes the reaction S P. The substrate concentration have been spaced to allow use of any of the linear plots. Plot the data to determine (15) Km and Vmax.

Q: A plot of enzyme activity with and without an inhibitor present gave the following. Use two…

A: The rates of biochemical reactions are increased by proteins known as enzymes. Inhibitors are…

Q: gluscose trasporter in teh liver cell binds with Km of 40mM. Calculate what fraction of vmax the Vo…

A: Km is the kinetic constant that determines the affinity of an enzyme to its substrate.Vmax is…

Q: Which graph would you use to graphically determine Rmax and KM? O 150 O Rmax = R (µmol · L-1• min−¹)…

A: Km is the catalytic constant which is equal to the substrate concentration at half of maximum…

Q: rformed a lactate dehydrogenase (LDH) assay multiple times e same. This graph shows the results…

A: An enzyme is a protein which is required for carrying out a particular chemical reaction inside the…

Q: (a) Estimate the Km and Vmax for the wild-type and mutant enzyme from the graph. (b) Calculate the…

A: For a one-substrate enzyme catalyzed reaction, the Michaelis-Menton equation shows the quantitative…

Q: The equation for the overall reaction that occurs in Complex I is shown below: NADH + H* +Q= NAD+ +…

A: Gibbs free energy is a measure of enthalpy taking entropy into account. It allows us to relate…

Q: With a AGO'of -16.7 kJ/mol, the reaction catalyzed by hexokinase is considered to be at equilibrium…

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Q: List 4 major types of inhibition modes and clearly indicate the effect on Vmax and KM for each mode?

A: “Since you have posted multiple questions, we will provide the solution only to the first question…

Q: 6. In the presence of a metabolite (X), 6-phosphofructokinase is assayed at a fixed concentration of…

A: Phosphofructokinase is an enzyme that takes part in the glycolytic pathway. It converts…

Q: V, (μmol-min-¹) 0.35 1/V₂ 0.3 0.25 0.2 0.15 0.1 0.05 0 0 50 100 150 200 250 300 350 400 450 [S] (nm)…

A: Michaelis Menten kinetics and Lineweaver Burk plot show the activity of the enzyme catalyzed…

Q: An enzyme catalyzes He veaction AZB, The en&yme is present at a Cencentration of 2nl, an Vmax s 22…

A: A study that deals with the reaction (conversion of substrate into the product) catalyzed by enzymes…

Q: (d) From your plot, calculate the activity of the enzyme. Use the space below to show your workings:

A: Enzyme kinetics - is the study of the reaction rate of enzyme catalyzed reactions. The reaction rate…

Q: For the reaction A + B = C + D, assume that the standard change in free energy has a positive value.…

A: Consider the following general reaction:aA + bB ⇌ cC + dDAt equilibrium (steady state), the…

Q: Suppose that you are working with an enzyme that has an ionizable active site residue with a pk of…

A: First let's name the curves as shown below, so that we can identify each of them using these…

Q: 2. The kinetics of an enzyme are measured as a function of substrate concentration in the presence…

A: Enzymes are catalysts that increase the rate of biochemical reactions. The inhibition of enzyme…

Q: What is the impact of the lower value Vmax on the affinity for enzyme for substrate? And what is…

A: The relationship between Km and substrate concentration is that Km corresponds to the substrate…

Q: g) Do some research. How does the level of phosphocreatine remain high in muscle cells. Where/how is…

A: Phosphocreatine (PCr) levels in muscle cells are maintained through a process called the creatine…

Q: 2C Two homologues of this enzyme from different species both catalyze the reaction SY with distinct…

A: Hi! Thank you for the question. We are authorized to answer one question at a time, since you have…

Q: 2.2

A: Lactose is a carbohydrate and it is also known as milk sugar. Lactose is a disaccharide composed of…

Q: 1. Suppose the following kinetic data was collected for an enzymatic reaction For each substrate…

A: Enzyme are proteins that catalyse the conversion of substrate into product. The mathematical…

Q: Based on the Lineweaver-Burke plot attached.  Kinetic data were generated in the (1) absence of any…

A: Given values: Y-intercept of line A and B = 0.9 sec/μM Y-intercept of C-line = 0.3 sec/μM

Q: The following data were collected for an enzyme that can be described by Michaelis Menton kinetics.…

A: In-order to get accurate Km and Vmax values, we need to draw the LB plot. The LB plot has 1V (i.e.…

Q: Based on the Lineweaver-Burke plot attached. Kinetic data were generated in the (1) absence of any…

A: Given Values: Y-intercept of the B line = 0.9 secμM Slope of B line = 0.6 sec

Q: 5. Please draw typical curves of the relationship between enzyme catalytic rate(v)and substrate…

A: Enzymes are proteins that catalyze biochemical reactions. Catalytic activity refers to the rate at…

Q: In the same kinetics experiment above without the inhibitor, what is the fraction of occupied enzyme…

A: Michaelis Menton equationFor a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton…

Q: The purpose of the progress curve is to determine if an enzymatic reaction rate remains constant for…

A: The progress curve is the measure of enzymatic velocity in a reaction. Velocity is given by the rate…

Q: Based on the Lineweaver-Burke plot attached.  Kinetic data were generated in the (1) absence of any…

A: Three datasets are plotted onto the line-weaver burk plot. One of the lines from A, B, and C…

Q: What enzyme kinetic parameters are apparantly impacted by uncompetitive inhibitors?     Vmax…

A: In UNCOMPETITIVE INHIBITION,  an inhibitor bind at a site distict from the substrate. However,…

Q: what is the relation between K'eq and mass action ratio (Q) for biochem? specifically what are the…

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Q: 2. The kinetics of an enzyme are measured as a function of substrate concentration in the presence…

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Q: assume that for an enzyme immobilized on the surface of a nonporous support material, the external…

A: Introduction  Enzymes are biological molecules, typically proteins, that act as catalysts for…

Q: 111 The Michaelis constant is represented by what variable, and is comprised of which rate constants…

A: Michaelis constant is a quite important term as it is used for determining the concentration of any…

Metabolic pathways

Metabolism is defined as the group of life-supporting, enzyme-mediated chemical reactions in organisms. A metabolic pathway is a set of these chemical reactions that involve anabolism (synthesis of complex biomolecules) and catabolism (breakdown of biomolecules into small molecules) that occur inside a cell. Metabolites are the reacting substances, products, and intermediate substances of an enzymatic process that are altered by a series of chemical events catalyzed by enzymes.