Which graph would you use to graphically determine Rmax and KM? O 150 O Rmax = R (µmol · L-1• min−¹) KM = 1/R (L.min/umol) 125 100 75 50 25 0 0 0.05 0.04 0.03 0.02 0.01 "C y-intercept 137 L 0.05 L 0.1 L 0.05 slope -0.00173 0.15 1/[S] (L/umol) y-intercept = 0.00609 L 0.1 slope 0.196 0.15 1/[S] (L/mol) 0.2 1 0.2 I 0.25 L 0.25 R (umol. L-1. min-¹) 1/R (L.min/umol) 150 H 125 100 75 50 H 25 0.05 H 0.04 0.03 0.02 0.01 0 0% 0 y-intercept 17.2 I 50 y-intercept = 0.0472 50 slope 0.579 100 [S] (umol/L) I 100 [S] (umol/L) 150 200 slope 1.95 x 10-4 150 Determine the maximum rate of the enzyme (Rmax), often called the maximum velocity (Vmax), and the Michaelis-Menten constant of the enzyme (KM). I 200 μmol L. min μmol L
Which graph would you use to graphically determine Rmax and KM? O 150 O Rmax = R (µmol · L-1• min−¹) KM = 1/R (L.min/umol) 125 100 75 50 25 0 0 0.05 0.04 0.03 0.02 0.01 "C y-intercept 137 L 0.05 L 0.1 L 0.05 slope -0.00173 0.15 1/[S] (L/umol) y-intercept = 0.00609 L 0.1 slope 0.196 0.15 1/[S] (L/mol) 0.2 1 0.2 I 0.25 L 0.25 R (umol. L-1. min-¹) 1/R (L.min/umol) 150 H 125 100 75 50 H 25 0.05 H 0.04 0.03 0.02 0.01 0 0% 0 y-intercept 17.2 I 50 y-intercept = 0.0472 50 slope 0.579 100 [S] (umol/L) I 100 [S] (umol/L) 150 200 slope 1.95 x 10-4 150 Determine the maximum rate of the enzyme (Rmax), often called the maximum velocity (Vmax), and the Michaelis-Menten constant of the enzyme (KM). I 200 μmol L. min μmol L
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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C7 Q9:
![Which graph would you use to graphically determine Rmax and KM?
O 150
O
Rmax =
R (µmol • L-1• min−¹)
KM =
1/R (L.min/μmol)
125
100
75
50
25
0
0
0.05
0.04
0.03
0.02
0.01
"C
y-intercept 137
L
0.05
L
0.1
L
0.05
slope -0.00173
0.15
1/[S] (Lumol)
y-intercept = 0.00609
L
0.1
slope 0.196
0.15
1/[S] (L/μmol)
0.2
1
0.2
I
0.25
L
0.25
R (umol. L-1. min-¹)
1/R (L• min/μmol)
150 H
125
100
75
50 H
25
0.05 H
0.04
0.03
0.02
0.01
0
0%
0
y-intercept 17.2
I
50
y-intercept = 0.0472
50
slope 0.579
100
[S] (umol/L)
I
100
[S] (µmol/L)
150
200
slope-1.95 x 10-4
150
Determine the maximum rate of the enzyme (Rmax), often called the maximum velocity (Vmax), and the Michaelis-Menten
constant of the enzyme (KM).
I
200
μmol
L. min
μmol
L](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc94cb5d1-997a-49d0-b0ef-9ec02bf0a187%2F17dd9a17-aed7-4fbe-8a26-573e660e0826%2Fi28vr14_processed.png&w=3840&q=75)
Transcribed Image Text:Which graph would you use to graphically determine Rmax and KM?
O 150
O
Rmax =
R (µmol • L-1• min−¹)
KM =
1/R (L.min/μmol)
125
100
75
50
25
0
0
0.05
0.04
0.03
0.02
0.01
"C
y-intercept 137
L
0.05
L
0.1
L
0.05
slope -0.00173
0.15
1/[S] (Lumol)
y-intercept = 0.00609
L
0.1
slope 0.196
0.15
1/[S] (L/μmol)
0.2
1
0.2
I
0.25
L
0.25
R (umol. L-1. min-¹)
1/R (L• min/μmol)
150 H
125
100
75
50 H
25
0.05 H
0.04
0.03
0.02
0.01
0
0%
0
y-intercept 17.2
I
50
y-intercept = 0.0472
50
slope 0.579
100
[S] (umol/L)
I
100
[S] (µmol/L)
150
200
slope-1.95 x 10-4
150
Determine the maximum rate of the enzyme (Rmax), often called the maximum velocity (Vmax), and the Michaelis-Menten
constant of the enzyme (KM).
I
200
μmol
L. min
μmol
L
![The data in the table was collected for a certain
enzyme-catalyzed reaction. Use these data to determine the
maximum rate of the enzyme (Rmax), often called the
maximum velocity (Vmax), and the Michaelis-Menten constant
of the enzyme (KM).
If you were to plot this data to graphically determine Rmax and
KM using a Lineweaver-Burk plot, what would you plot? If
you would use the original data in the table, enter the original
value for your answer.
[S] = 5.0 µmol-L-¹: Graph as
R = 22 µmol-L-¹-min¹: Graph as
[S] = 20.0 µmol-L-¹: Graph as
R = 65 µmol·L-¹-min-¹: Graph as
Which graph would you use to graphically determine Rmax and KM?
L-1.min-1)
150-
125
100
75
[S] (μmol-L-¹)
5.0
10.0
20.0
50.0
100.0
200.0
y-intercept - 137
L-¹. min-¹)
150 H
125
100
75
R (μmol-L-¹-min¯¹)
22
39
65
102
120
135
slope = 0.579](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc94cb5d1-997a-49d0-b0ef-9ec02bf0a187%2F17dd9a17-aed7-4fbe-8a26-573e660e0826%2F69p2ou_processed.png&w=3840&q=75)
Transcribed Image Text:The data in the table was collected for a certain
enzyme-catalyzed reaction. Use these data to determine the
maximum rate of the enzyme (Rmax), often called the
maximum velocity (Vmax), and the Michaelis-Menten constant
of the enzyme (KM).
If you were to plot this data to graphically determine Rmax and
KM using a Lineweaver-Burk plot, what would you plot? If
you would use the original data in the table, enter the original
value for your answer.
[S] = 5.0 µmol-L-¹: Graph as
R = 22 µmol-L-¹-min¹: Graph as
[S] = 20.0 µmol-L-¹: Graph as
R = 65 µmol·L-¹-min-¹: Graph as
Which graph would you use to graphically determine Rmax and KM?
L-1.min-1)
150-
125
100
75
[S] (μmol-L-¹)
5.0
10.0
20.0
50.0
100.0
200.0
y-intercept - 137
L-¹. min-¹)
150 H
125
100
75
R (μmol-L-¹-min¯¹)
22
39
65
102
120
135
slope = 0.579
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