he Lineweaver-Burk plot, which illustrates the reciprocal of the reaction rate (1/v) versus the reciprocal of the substrate oncentration (1/[S]), is a graphical representation of enzyme kinetics. This plot is typically used to determine the maximum ate, Vmax, and the Michaelis constant, Km, which can be gleaned from the intercepts and slope. Identify each intercept and the lope in terms of the constants Vmax and Km. x-intercept Lineweaver-Burk Plot 1/v ..y-intercept Slope 1/[S] -1/Km 1/Km Km/Vmax 1/Vmax Answer Bank Vmax -Vmax -Km/Vmax -1/Vmax -Km Vmax/Km -Vmax/Km Km

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Chapter1: Biochemistry: An Evolving Science
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C7 Q8:

The Lineweaver-Burk plot, which illustrates the reciprocal of the reaction rate (1/v) versus the reciprocal of the substrate
concentration (1/[S]), is a graphical representation of enzyme kinetics. This plot is typically used to determine the maximum
rate, Vmax, and the Michaelis constant, Km, which can be gleaned from the intercepts and slope. Identify each intercept and the
slope in terms of the constants Vmax and Km.
x-intercept
Lineweaver-Burk Plot
1/v
y-intercept
Slope
1/[S]
-1/Km
1/Km
Km/Vmax
1/Vmax
Answer Bank
Vmax
-Km/Vmax
-1/Vmax
-Vmax
-Km
Vmax/Km
-Vmax/km
Km
Transcribed Image Text:The Lineweaver-Burk plot, which illustrates the reciprocal of the reaction rate (1/v) versus the reciprocal of the substrate concentration (1/[S]), is a graphical representation of enzyme kinetics. This plot is typically used to determine the maximum rate, Vmax, and the Michaelis constant, Km, which can be gleaned from the intercepts and slope. Identify each intercept and the slope in terms of the constants Vmax and Km. x-intercept Lineweaver-Burk Plot 1/v y-intercept Slope 1/[S] -1/Km 1/Km Km/Vmax 1/Vmax Answer Bank Vmax -Km/Vmax -1/Vmax -Vmax -Km Vmax/Km -Vmax/km Km
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