Predict and explain the effect on GAL1 transcription, in the presence of galactose alone, of the following mutations:a. Deletion of one Gal4-binding site in the GAL1 UAS element.b. Deletion of all four Gal4-binding sites in the GAL1 UAS element.c. Deletion of the Mig1-binding site upstream of GAL1.d. Deletion of the Gal4 activation domain.e. Deletion of the GAL80 gene.f. Deletion of the GAL1 promoter.g. Deletion of the GAL3 gene.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Predict and explain the effect on GAL1 transcription, in
the presence of galactose alone, of the following
mutations:
a. Deletion of one Gal4-binding site in the GAL1 UAS
element.
b. Deletion of all four Gal4-binding sites in the GAL1
UAS element.
c. Deletion of the Mig1-binding site upstream of GAL1.
d. Deletion of the Gal4 activation domain.
e. Deletion of the GAL80 gene.
f. Deletion of the GAL1 promoter.
g. Deletion of the GAL3 gene.
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