2. (A) Describe the role that PI plays in transcriptional regulation of the ginALG operon and in the regulation of activity of glutamine synthetase. How are the levels of PI and Pu-UMP regulated? How does this system operate to globally regulate nitrogen assimilation? Answer all parts of this question. (B.) What is FNR and what is its role? How does it function? How is it similar to CAP (or CRP)? (C) The Hut (Histidine Utilization) and Put (Proline Utilization) operons are globally regulated by both CAP (catabolite activator protein) and NTR (nitrogen regulatory protein). How and Why? OR
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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