Let’s suppose a researcher was interested in the effects of mutationson the expression of a protein-encoding gene for a proteinthat is 472 amino acids in length. This protein is expressed in leafcells of Arabidopsis thaliana. It has a molecular mass of approximately56,640 Da. Make a drawing that shows the expected resultsof a Western blot using proteins isolated from the leaf cells thatwere obtained from the following plants:Lane 1. A plant homozygous for a nonmutant geneLane 2. A plant homozygous for a deletion that removes the promoterfor this geneLane 3. A heterozygous plant in which one gene is nonmutant andthe other gene has a mutation that introduces an early stop codon atcodon 112Lane 4. A plant homozygous for a mutation that introduces anearly stop codon at codon 112Lane 5. A plant homozygous for a mutation that changes codon108 from a phenylalanine codon into a leucine codon
Let’s suppose a researcher was interested in the effects of mutations
on the expression of a protein-encoding gene for a protein
that is 472 amino acids in length. This protein is expressed in leaf
cells of Arabidopsis thaliana. It has a molecular mass of approximately
56,640 Da. Make a drawing that shows the expected results
of a Western blot using proteins isolated from the leaf cells that
were obtained from the following plants:
Lane 1. A plant homozygous for a nonmutant gene
Lane 2. A plant homozygous for a deletion that removes the promoter
for this gene
Lane 3. A heterozygous plant in which one gene is nonmutant and
the other gene has a mutation that introduces an early stop codon at
codon 112
Lane 4. A plant homozygous for a mutation that introduces an
early stop codon at codon 112
Lane 5. A plant homozygous for a mutation that changes codon
108 from a phenylalanine codon into a leucine codon
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