Answered: The Saccharomyces cerevisiae nuclear…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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The Saccharomyces cerevisiae nuclear gene ARG8
encodes an enzyme that catalyzes a key step in biosynthesis of the amino acid arginine. This protein is
normally synthesized on cytoplasmic ribosomes, but
then is transported into mitochondria, where the enzyme conducts its functions. In 1996, T. D. Fox and
his colleagues constructed a strain of yeast in which a
gene encoding the Arg8 protein was itself moved into
mitochondria, where functional protein could be
synthesized on mitochondrial ribosomes.
a. How could these investigators move the ARG8
gene from the nucleus into the mitochondria, while
permitting the synthesis of active enzyme? In what
ways would the investigators need to alter the
ARG8 gene to allow it to function in the mitochondria instead of in the nucleus?
b. Why might these researchers have wished to move
the ARG8 gene into mitochondria in the first
place?

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