When 1 million cells of a culture of haploid yeastcarrying a met− auxotrophic mutation were plated onpetri plates lacking methionine (Met), five coloniesgrew. You would expect cells in which the originalmet− mutation was reversed (by a base change back tothe original sequence) would grow on the media lackingmethionine, but some of these apparent reversions couldbe due to a mutation in a different gene that somehowsuppresses the original met− mutations. How wouldyou be able to determine if the mutations in your fivecolonies were due either to a precise reversion of theoriginal met− mutation or to the generation of a suppressor mutation in a gene on another chromosome?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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When 1 million cells of a culture of haploid yeast
carrying a met− auxotrophic mutation were plated on
petri plates lacking methionine (Met), five colonies
grew. You would expect cells in which the original
met− mutation was reversed (by a base change back to
the original sequence) would grow on the media lacking
methionine, but some of these apparent reversions could
be due to a mutation in a different gene that somehow
suppresses the original met− mutations. How would
you be able to determine if the mutations in your five
colonies were due either to a precise reversion of the
original met− mutation or to the generation of a suppressor mutation in a gene on another chromosome?

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