“DNMT3L Connects Unmethylated Lysine 4 of Histone H3 to de novo Methylation of DNA,” the authors determined that the DNMT3L protein interacts with several histone proteins.
DNA and RNA
Deoxyribonucleic acid (DNA) is usually called the blueprint of life. Deoxyribose is a monosaccharide that has a key function in the synthesis of deoxyribonucleic acid. One less oxygen-containing hydroxyl group occurs in deoxyribose sugar. Nucleic acid, deoxyribonucleic acid, is one of the natural components. Deoxyribonucleic acid is a double-stranded molecule. Watson and Crick postulated the double-stranded model of the helix. A deoxyribonucleic acid is a molecular group that carries and transmits genetic information from parents to offspring. All eukaryotic and prokaryotic cells are involved.
DNA as the Genetic Material
DNA, or deoxyribonucleic acid, is a long polymeric nucleic acid molecule discovered in the late 1930s. It is a polymer; a long chain-like molecule made up of several monomers connected in a sequence. It possesses certain characteristics that qualify it as a genetic component. Certain organisms have different types of nucleic acids as their genetic material - DNA or RNA.
Genetics
The significant branch in science which involves the study of genes, gene variations, and the organism's heredity is known as genetics. It is also used to study the involvement of a gene or set of genes in the health of an individual and how it prevents several diseases in a human being. Thus, genetics also creates an understanding of various medical conditions.
DNA Replication
The mechanism by which deoxyribonucleic acid (DNA) is capable of producing an exact copy of its own is defined as DNA replication. The DNA molecules utilize a semiconservative method for replication.
In the first figure of “DNMT3L Connects Unmethylated Lysine 4 of Histone H3 to de novo Methylation of DNA,” the authors determined that the DNMT3L protein interacts with several histone proteins. Using Figure a and b (from that paper), explain how each of the 4 histones interact with DNMT3L.
![a
Wild type
Tag +
Neo
Tag
Exon: 12 34
"I
ATG
56 7 8
H
HH Pgk1-Neo
ATG
| Cre
Y
HHHHHH
MGSR-His-FLAG
9
+
H
+
10 11
H
loxP site
H
12
2 kb
Figure 1 Generation of epitope-tagged Dnmt3L locus and DNMT3L
protein interaction screen in ES cells. a, Targeting of the endogenous mouse
Dnmt3L locus with a replacement cassette that introduces an N-terminal
His6-FLAG epitope tag. The tag was introduced immediately after the first
four amino acids of DNMT3L (Supplementary Fig. S1). The neo resistance
cassette was deleted by transient exposure to Cre recombinase. Mice
homozygous for the Dnmt3LT8 allele are fertile and were used to generate
homozygous Dnmt3LTag/Tag ES cells. The southern blot on the right shows
13
요
8-
6-
5-
Tag +
Neo
- Tag
-Wild type
b
M, x 10-³
83-
62-
47.5-
32.5-
25-
16.5-
6.5-
+/+ Tag/Tag
DNMT3A2
DNMT3B
-DNMT3L
H3
H2A/H2B
-H4
that homologous recombination and Cre-mediated excision of the marker
occurred as predicted. b, Protein interaction screen in wild-type and
Dnmt31 Tag/Tag p
ES cells by FLAG immunoprecipitation. Coomassie-stained
bands were subjected to MALDI-reTOF and mass spectra were screened
against a non-redundant protein database to identify interacting proteins.
The protein band marked by the asterisk is actin, a common contaminant in
anti-FLAG immunoprecipitation.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fd5efe18d-e46d-434a-8979-3f042834e5cd%2F3a56532b-21a6-4c43-9a42-593769930775%2Fsw6o90p_processed.png&w=3840&q=75)
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